Development of a Semiquantitative Degenerate Real-time Pcr-based Assay for Estimation of Numbers of Butyryl-coenzyme A (CoA) CoA Transferase Genes in Complex Bacterial Samples

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2007 Jan 30

PMID 17259367

Citations 89

Authors

Petra Louis

Harry J Flint

Affiliations

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Abstract

We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.

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