Gene Expression Profiling of Zebrafish Embryonic Retinal Pigment Epithelium in Vivo

Overview

Journal Invest Ophthalmol Vis Sci

Specialty Ophthalmology

Date 2007 Jan 26

PMID 17251491

Citations 16

Authors

Yuk Fai Leung

Ping Ma

John E Dowling

Affiliations

Soon will be listed here.

Abstract

Purpose: Eye development and photoreceptor maintenance is dependent on the retinal pigment epithelium (RPE), a thin layer of cells that underlies the neural retina. Despite its importance, development of RPE has not been studied by a genomic approach. In this study, a microarray expression-profiling methodology was established for studying RPE development.

Methods: The intact retina with RPE attached was dissected from developing embryos, and differentially expressed genes in RPE were inferred by comparing the dissected tissues with retinas without RPE, in microarray and statistical analyses.

Results: Of the probesets used, 8810 were significantly expressed in RPE at 52 hours postfertilization (hpf), of which 1443 may have biologically meaningful expression levels. Further, 78 and 988 probesets were found to be significantly over- or underexpressed in RPE, respectively, compared with retina. Also, 79.2% (38/48) of the known overexpressed probesets were independently validated as RPE-related transcripts.

Conclusions: The results strongly suggest that this methodology can obtain in vivo RPE-specific gene expression from the zebrafish embryos and identify novel RPE markers.

Citing Articles

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Zhang L, Ma P, Collery R, Trowbridge S, Zhang M, Zhong W Mol Vis. 2014; 20:56-72.

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