New Map Location of IlvO in ESCHERICHIA COLI

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1976 Jun 1

PMID 17248710

Citations 20

Authors

B M Cohen

E W Jones

Affiliations

Soon will be listed here.

Abstract

Mutations in ilvO, the operator site for operon A of the ilv region of E. coli K-12, are reported to cause a cis dominant derepression of the operon A gene products and to map between ilvC and ilvA. Studies reported here demonstrate that the F25 episome which does not carry the region between ilvC and ilvA but only ilvE, ilvD and a part of ilvA, can transfer ilvO genetic material. Also, genes of operon A on the F25 episome respond normally to a derepression signal. It is proposed that ilvO is on the F25 episome. Five-point crosses demonstrate that mutations in ilvO map nearer to ilvE than to ilvA. Considering this and other evidence, it is proposed that the gene order in the ilv region is CADEO, not COADE, with transcription and translation in operon A being from ilvE to ilvA not ilvA to ilvE. It is confirmed that mutations in ilvO cause a cis dominant derepression of the operon A gene products but it is also noted that mutations in ilvO lead to the appearance of an AHAS activity more resistant to inhibition by valine than that of the present O(+) strain.

Citing Articles

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Molecular basis of valine resistance in Escherichia coli K-12.

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PMID: 7015336 PMC: 319916. DOI: 10.1073/pnas.78.2.922.

Linkage map of Escherichia coli K-12, edition 6.

Bachmann B, Low K Microbiol Rev. 1980; 44(1):1-56.

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Genetical and structural analysis of a group of lambda ilv and lambda rho transducing phages.

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Mutagenesis by insertion of drug resistance transposon Tn7 into a vibrio species.

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