A Transgenic Mouse Model Engineered to Investigate Human Brain-derived Neurotrophic Factor in Vivo

Overview

Journal Transgenic Res

Specialty Molecular Biology

Date 2007 Jan 17

PMID 17225071

Citations 7

Authors

Fabrice Guillemot

Italina Cerutti

Charles Auffray

Marie-Dominique Devignes

Affiliations

Soon will be listed here.

Abstract

Brain-derived neurotrophic factor (BDNF) is an attractive component for the treatment of various neurodegenerative diseases such as Alzheimer's or Parkinson's disease. Innovative non-invasive therapeutic approaches involve appropriate pharmacological induction of endogenous BDNF synthesis in brain. A transgenic mouse model has been established to study human BDNF gene expression and permit the screening of compounds capable of stimulating its activity. A 145-kb yeast artificial chromosome carrying the human BDNF gene has been engineered to produce the transgene which contains the extended BDNF promoter and 3' flanking regions and has integrated the enhanced green fluorescent protein (E-GFP) coding sequence in place of the BDNF coding exon. Five transgenic lines have been obtained through microinjection of the YAC into fertilized mouse oocytes. From the three lines expressing the transgene, one displays the specific pattern of BDNF expression. Faithful tissue-restricted transcription of BDNF 5' exons and localization of the fluorescent reporter gene product in the expected brain subregions are reported. This line constitutes an exploitable system for investigating human BDNF gene regulation in vivo.

Citing Articles

A New Mouse Line Reporting the Translation of Brain-Derived Neurotrophic Factor Using Green Fluorescent Protein.

Wosnitzka E, Nan X, Nan J, Chacon-Fernandez P, Kussmaul L, Schuler M eNeuro. 2019; 7(1).

PMID: 31882533 PMC: 6957309. DOI: 10.1523/ENEURO.0462-19.2019.

BDNF-Live-Exon-Visualization (BLEV) Allows Differential Detection of BDNF Transcripts and .

Singer W, Manthey M, Panford-Walsh R, Matt L, Geisler H, Passeri E Front Mol Neurosci. 2018; 11:325.

PMID: 30319348 PMC: 6170895. DOI: 10.3389/fnmol.2018.00325.

Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice.

Fukuchi M, Izumi H, Mori H, Kiyama M, Otsuka S, Maki S Sci Rep. 2017; 7(1):4949.

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Pharmacological profile of brain-derived neurotrophic factor (BDNF) splice variant translation using a novel drug screening assay: a "quantitative code".

Vaghi V, Polacchini A, Baj G, Pinheiro V, Vicario A, Tongiorgi E J Biol Chem. 2014; 289(40):27702-13.

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BAC-based cellular model for screening regulators of BDNF gene transcription.

Jaanson K, Sepp M, Aid-Pavlidis T, Timmusk T BMC Neurosci. 2014; 15:75.

PMID: 24943717 PMC: 4071165. DOI: 10.1186/1471-2202-15-75.

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