Investigating Membrane Protein Dynamics in Living Cells

Overview

Journal Biochem Cell Biol

Specialties Biochemistry
Cell Biology

Date 2007 Jan 12

PMID 17215870

Citations 15

Authors

Ian R Bates

Paul W Wiseman

John W Hanrahan

Affiliations

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Abstract

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

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