Co-evolution of the Archaeal TRNA-dependent Amidotransferase GatCAB with TRNA(Asn)

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2007 Jan 12

PMID 17214986

Citations 10

Authors

Suk Namgoong

Kelly Sheppard

R Lynn Sherrer

Dieter Soll

Affiliations

Soon will be listed here.

Abstract

The important identity elements in tRNA(Gln) and tRNA(Asn) for bacterial GatCAB and in tRNA(Gln) for archaeal GatDE are the D-loop and the first base pair of the acceptor stem. Here we show that Methanothermobacter thermautotrophicus GatCAB, the archaeal enzyme, is different as it discriminates Asp-tRNA(Asp) and Asp-tRNA(Asn) by use of U49, the D-loop and to a lesser extent the variable loop. Since archaea possess the tRNA(Gln)-specific amidotransferase GatDE, the archaeal GatCAB enzyme evolved to recognize different elements in tRNA(Asn) than those recognized by GatDE or by the bacterial GatCAB enzyme in their tRNA substrates.

Citing Articles

Unconventional genetic code systems in archaea.

Meng K, Chung C, Soll D, Krahn N Front Microbiol. 2022; 13:1007832.

PMID: 36160229 PMC: 9499178. DOI: 10.3389/fmicb.2022.1007832.

Identifying the ligated amino acid of archaeal tRNAs based on positions outside the anticodon.

Galili T, Gingold H, Shaul S, Benjamini Y RNA. 2016; 22(10):1477-91.

PMID: 27516383 PMC: 5029447. DOI: 10.1261/rna.053777.115.

The archaeal transamidosome for RNA-dependent glutamine biosynthesis.

Rampias T, Sheppard K, Soll D Nucleic Acids Res. 2010; 38(17):5774-83.

PMID: 20457752 PMC: 2943598. DOI: 10.1093/nar/gkq336.

Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition.

Nakamura A, Sheppard K, Yamane J, Yao M, Soll D, Tanaka I Nucleic Acids Res. 2009; 38(2):672-82.

PMID: 19906721 PMC: 2811023. DOI: 10.1093/nar/gkp955.

Recognition of tRNAGln by Helicobacter pylori GluRS2--a tRNAGln-specific glutamyl-tRNA synthetase.

Chang K, Hendrickson T Nucleic Acids Res. 2009; 37(20):6942-9.

PMID: 19755501 PMC: 2777447. DOI: 10.1093/nar/gkp754.

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