Cytomegalovirus Primary Envelopment Occurs at Large Infoldings of the Inner Nuclear Membrane

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2006 Dec 29

PMID 17192309

Citations 39

Authors

Christopher Buser

Paul Walther

Thomas Mertens

Detlef Michel

Affiliations

Soon will be listed here.

Abstract

We have investigated the morphogenesis of human and murine cytomegalovirus by transmission electron microscopy after high-pressure freezing, freeze substitution, and plastic embedding. We observed large tubular infoldings of the inner nuclear membrane that were free of lamina and active in primary envelopment and subsequent transport of capsids to the nuclear periphery. Semiquantitative determinations of the enlarged inner nuclear membrane area and the location of the primary envelopment of nucleocapsids demonstrated that this structure represents a virus-induced specialized membrane domain at which the particles are preferentially enveloped. This is a previously undescribed structural element relevant in cytomegalovirus morphogenesis.

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