Effect of Long-term Exposure of SH-SY5Y Cells to Morphine: a Whole Cell Proteomic Analysis

Overview

Journal Proteome Sci

Publisher Biomed Central

Date 2006 Dec 23

PMID 17184524

Citations 7

Authors

Jeremie Neasta

Sandrine Uttenweiler-Joseph

Karima Chaoui

Bernard Monsarrat

Jean-Claude Meunier

Lionel Mouledous

Affiliations

Soon will be listed here.

Abstract

Background: Opiate addiction reflects plastic changes that endurably alter synaptic transmission within relevant neuronal circuits. The biochemical mechanisms of these adaptations remain largely unknown and proteomics-based approaches could lead to a broad characterization of the molecular events underlying adaptations to chronic drug exposure.

Results: Thus, we have started proteomic analyses of the effects of chronic morphine exposure in a recombinant human neuroblastoma SH-SY5Y clone that stably overexpresses the mu-opioid receptor. Cells were treated with morphine for 6, 24 and 72 hours, the proteins were separated by 2-D gel electrophoresis and stained with Coomassie blue, and the protein map was compared with that obtained from untreated cells. Spots showing a statistically significant variation were selected for identification using mass spectrometric analyses.

Conclusion: A total of 45 proteins were identified, including proteins involved in cellular metabolism, cytoskeleton organization, vesicular trafficking, transcriptional and translational regulation, and cell signaling.

Citing Articles

The ubiquitin-proteasome system and learning-dependent synaptic plasticity - A 10 year update.

Patrick M, Omar N, Werner C, Mitra S, Jarome T Neurosci Biobehav Rev. 2023; 152:105280.

PMID: 37315660 PMC: 11323321. DOI: 10.1016/j.neubiorev.2023.105280.

Roles of the ubiquitin proteasome system in the effects of drugs of abuse.

Massaly N, Frances B, Mouledous L Front Mol Neurosci. 2015; 7:99.

PMID: 25610367 PMC: 4285073. DOI: 10.3389/fnmol.2014.00099.

Global changes in the rat heart proteome induced by prolonged morphine treatment and withdrawal.

Drastichova Z, Skrabalova J, Jedelsky P, Neckar J, Kolar F, Novotny J PLoS One. 2012; 7(10):e47167.

PMID: 23056601 PMC: 3467212. DOI: 10.1371/journal.pone.0047167.

Morphine produces immunosuppressive effects in nonhuman primates at the proteomic and cellular levels.

Brown J, Ortiz G, Angel T, Jacobs J, Gritsenko M, Chan E Mol Cell Proteomics. 2012; 11(9):605-18.

PMID: 22580588 PMC: 3434775. DOI: 10.1074/mcp.M111.016121.

Genes and pathways co-associated with the exposure to multiple drugs of abuse, including alcohol, amphetamine/methamphetamine, cocaine, marijuana, morphine, and/or nicotine: a review of proteomics analyses.

Wang J, Yuan W, Li M Mol Neurobiol. 2011; 44(3):269-86.

PMID: 21922273 DOI: 10.1007/s12035-011-8202-4.

References

Xu N, Yu Y, Zhu J, Liu H, Shen L, Zeng R . Inhibition of SNAP-25 phosphorylation at Ser187 is involved in chronic morphine-induced down-regulation of SNARE complex formation. J Biol Chem. 2004; 279(39):40601-8. DOI: 10.1074/jbc.M406896200. View

Williams K, Wu T, Colangelo C, Nairn A . Recent advances in neuroproteomics and potential application to studies of drug addiction. Neuropharmacology. 2004; 47 Suppl 1:148-66. DOI: 10.1016/j.neuropharm.2004.07.009. View

Cheng L, Itoh K, Lemmon V . L1-mediated branching is regulated by two ezrin-radixin-moesin (ERM)-binding sites, the RSLE region and a novel juxtamembrane ERM-binding region. J Neurosci. 2005; 25(2):395-403. PMC: 2860578. DOI: 10.1523/JNEUROSCI.4097-04.2005. View

Liao D, Lin H, Law P, Loh H . Mu-opioid receptors modulate the stability of dendritic spines. Proc Natl Acad Sci U S A. 2005; 102(5):1725-30. PMC: 545084. DOI: 10.1073/pnas.0406797102. View

Kim S, Chudapongse N, Lee S, Levin M, Oh J, Park H . Proteomic analysis of phosphotyrosyl proteins in morphine-dependent rat brains. Brain Res Mol Brain Res. 2005; 133(1):58-70. DOI: 10.1016/j.molbrainres.2004.09.018. View

Rhodes J, Crabbe J . Gene expression induced by drugs of abuse. Curr Opin Pharmacol. 2005; 5(1):26-33. DOI: 10.1016/j.coph.2004.12.001. View

Prokai L, Zharikova A, Stevens Jr S . Effect of chronic morphine exposure on the synaptic plasma-membrane subproteome of rats: a quantitative protein profiling study based on isotope-coded affinity tags and liquid chromatography/mass spectrometry. J Mass Spectrom. 2005; 40(2):169-75. DOI: 10.1002/jms.736. View

Mouledous L, Neasta J, Uttenweiler-Joseph S, Stella A, Matondo M, Corbani M . Long-term morphine treatment enhances proteasome-dependent degradation of G beta in human neuroblastoma SH-SY5Y cells: correlation with onset of adenylate cyclase sensitization. Mol Pharmacol. 2005; 68(2):467-76. DOI: 10.1124/mol.105.013391. View

Goody R, Rak A, Alexandrov K . The structural and mechanistic basis for recycling of Rab proteins between membrane compartments. Cell Mol Life Sci. 2005; 62(15):1657-70. PMC: 11139110. DOI: 10.1007/s00018-005-4486-8. View

10.

McClung C, Nestler E, Zachariou V . Regulation of gene expression by chronic morphine and morphine withdrawal in the locus ceruleus and ventral tegmental area. J Neurosci. 2005; 25(25):6005-15. PMC: 6724795. DOI: 10.1523/JNEUROSCI.0062-05.2005. View

11.

Kumar A, Choi K, Renthal W, Tsankova N, Theobald D, Truong H . Chromatin remodeling is a key mechanism underlying cocaine-induced plasticity in striatum. Neuron. 2005; 48(2):303-14. DOI: 10.1016/j.neuron.2005.09.023. View

12.

Seong H, Jung H, Choi H, Kim K, Ha H . Regulation of transforming growth factor-beta signaling and PDK1 kinase activity by physical interaction between PDK1 and serine-threonine kinase receptor-associated protein. J Biol Chem. 2005; 280(52):42897-908. DOI: 10.1074/jbc.M507539200. View

13.

Koob G, Moal M . Plasticity of reward neurocircuitry and the 'dark side' of drug addiction. Nat Neurosci. 2005; 8(11):1442-4. DOI: 10.1038/nn1105-1442. View

14.

Rambhia S, Mantione K, Stefano G, Cadet P . Morphine modulation of the ubiquitin-proteasome complex is neuroprotective. Med Sci Monit. 2005; 11(11):BR386-96. View

15.

Ron D, Jurd R . The "ups and downs" of signaling cascades in addiction. Sci STKE. 2005; 2005(309):re14. DOI: 10.1126/stke.3092005re14. View

16.

Li K, Jimenez C, van der Schors R, Hornshaw M, Schoffelmeer A, Smit A . Intermittent administration of morphine alters protein expression in rat nucleus accumbens. Proteomics. 2006; 6(6):2003-8. DOI: 10.1002/pmic.200500045. View

17.

Bierczynska-Krzysik A, Bonar E, Drabik A, Noga M, Suder P, Dylag T . Rat brain proteome in morphine dependence. Neurochem Int. 2006; 49(4):401-6. DOI: 10.1016/j.neuint.2006.01.024. View

18.

Kennedy M, Ehlers M . Organelles and trafficking machinery for postsynaptic plasticity. Annu Rev Neurosci. 2006; 29:325-62. PMC: 1876664. DOI: 10.1146/annurev.neuro.29.051605.112808. View

19.

Kazmi S, Mishra R . Comparative pharmacological properties and functional coupling of mu and delta opioid receptor sites in human neuroblastoma SH-SY5Y cells. Mol Pharmacol. 1987; 32(1):109-18. View

20.

Threadgill R, Bobb K, Ghosh A . Regulation of dendritic growth and remodeling by Rho, Rac, and Cdc42. Neuron. 1997; 19(3):625-34. DOI: 10.1016/s0896-6273(00)80376-1. View