31P MR Spectroscopy and in Vitro Markers of Oxidative Capacity in Type 2 Diabetes Patients

Overview

Journal MAGMA

Publisher Springer

Date 2006 Dec 21

PMID 17180611

Citations 11

Authors

S F E Praet

H M M De Feyter

R A M Jonkers

K Nicolay

C van Pul

H Kuipers

L J C van Loon

J J Prompers

Affiliations

Soon will be listed here.

Abstract

Background: Skeletal muscle mitochondrial function in type 2 diabetes (T2D) is currently being studied intensively. In vivo (31)P magnetic resonance spectroscopy ((31)P MRS) is a noninvasive tool used to measure mitochondrial respiratory function (MIFU) in skeletal muscle tissue. However, microvascular co-morbidity in long-standing T2D can interfere with the (31)P MRS methodology.

Aim: To compare (31)P MRS-derived parameters describing in vivo MIFU with an in vitro assessment of muscle respiratory capacity and muscle fiber-type composition in T2D patients.

Methods: (31)P MRS was applied in long-standing, insulin-treated T2D patients. (31)P MRS markers of MIFU were measured in the M. vastus lateralis. Muscle biopsy samples were collected from the same muscle and analyzed for succinate dehydrogenase activity (SDH) and fiber-type distribution.

Results: Several (31)P MRS parameters of MIFU showed moderate to good correlations with the percentage of type I fibers and type I fiber-specific SDH activity (Pearson's R between 0.70 and 0.75). In vivo and in vitro parameters of local mitochondrial respiration also correlated well with whole-body fitness levels (VO (2peak)) in these patients (Pearson's R between 0.62 and 0.90).

Conclusion: Good correlations exist between in vivo and in vitro measurements of MIFU in long-standing insulin-treated T2D subjects, which are qualitatively and quantitatively consistent with previous results measured in healthy subjects. This justifies the use of (31)P MRS to measure MIFU in relation to T2D.

Citing Articles

Clinical physiology: the crucial role of MRI in evaluation of peripheral artery disease.

Elsaid N, Peters D, Galiana G, Sinusas A Am J Physiol Heart Circ Physiol. 2024; 326(5):H1304-H1323.

PMID: 38517227 PMC: 11381027. DOI: 10.1152/ajpheart.00533.2023.

Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model.

Michel C, Messonnier L, Giannesini B, Chatel B, Vilmen C, Fur Y Front Physiol. 2022; 13:915640.

PMID: 35784862 PMC: 9240423. DOI: 10.3389/fphys.2022.915640.

Quantification of Mitochondrial Oxidative Phosphorylation in Metabolic Disease: Application to Type 2 Diabetes.

Lewis M, Kasper J, Bazil J, Frisbee J, Wiseman R Int J Mol Sci. 2019; 20(21).

PMID: 31652915 PMC: 6862501. DOI: 10.3390/ijms20215271.

Skeletal muscle performance in metabolic disease: Microvascular or mitochondrial limitation or both?.

Frisbee J, Lewis M, Wiseman R Microcirculation. 2018; 26(5):e12517.

PMID: 30471168 PMC: 6534486. DOI: 10.1111/micc.12517.

Reduced skeletal muscle phosphocreatine concentration in type 2 diabetic patients: a quantitative image-based phosphorus-31 MR spectroscopy study.

Ripley E, Clarke G, Hamidi V, Martinez R, Settles F, Solis C Am J Physiol Endocrinol Metab. 2018; 315(2):E229-E239.

PMID: 29509433 PMC: 6139498. DOI: 10.1152/ajpendo.00426.2017.

References

Mattei J, Bendahan D, Cozzone P . P-31 magnetic resonance spectroscopy. A tool for diagnostic purposes and pathophysiological insights in muscle diseases. Reumatismo. 2004; 56(1):9-14. DOI: 10.4081/reumatismo.2004.9. View

Massie B, Conway M, Yonge R, Frostick S, Sleight P, Ledingham J . 31P nuclear magnetic resonance evidence of abnormal skeletal muscle metabolism in patients with congestive heart failure. Am J Cardiol. 1987; 60(4):309-15. DOI: 10.1016/0002-9149(87)90233-5. View

Alberti K, Zimmet P . Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med. 1998; 15(7):539-53. DOI: 10.1002/(SICI)1096-9136(199807)15:7<539::AID-DIA668>3.0.CO;2-S. View

Larson-Meyer D, Newcomer B, Hunter G, Joanisse D, Weinsier R, Bamman M . Relation between in vivo and in vitro measurements of skeletal muscle oxidative metabolism. Muscle Nerve. 2001; 24(12):1665-76. DOI: 10.1002/mus.1202. View

Lodi R, Kemp G, Iotti S, Radda G, Barbiroli B . Influence of cytosolic pH on in vivo assessment of human muscle mitochondrial respiration by phosphorus magnetic resonance spectroscopy. MAGMA. 1997; 5(2):165-71. DOI: 10.1007/BF02592248. View

Bendahan D, Confort-Gouny S, Kozak-Reiss G, Cozzone P . Heterogeneity of metabolic response to muscular exercise in humans. New criteria of invariance defined by in vivo phosphorus-31 NMR spectroscopy. FEBS Lett. 1990; 272(1-2):155-8. DOI: 10.1016/0014-5793(90)80472-u. View

Young J, Pendergast D, Steinbach J . Oxygen transport and peripheral microcirculation in long-term diabetes. Proc Soc Exp Biol Med. 1991; 196(1):61-8. DOI: 10.3181/00379727-196-43164. View

Vanhamme , van den Boogaart A , Van Huffel S . Improved method for accurate and efficient quantification of MRS data with use of prior knowledge . J Magn Reson. 1998; 129(1):35-43. DOI: 10.1006/jmre.1997.1244. View

Iotti S, Lodi R, Frassineti C, Zaniol P, Barbiroli B . In vivo assessment of mitochondrial functionality in human gastrocnemius muscle by 31P MRS. The role of pH in the evaluation of phosphocreatine and inorganic phosphate recoveries from exercise. NMR Biomed. 1993; 6(4):248-53. DOI: 10.1002/nbm.1940060404. View

10.

Argov Z, De Stefano N, Arnold D . ADP recovery after a brief ischemic exercise in normal and diseased human muscle--a 31P MRS study. NMR Biomed. 1996; 9(4):165-72. DOI: 10.1002/(SICI)1099-1492(199606)9:4<165::AID-NBM408>3.0.CO;2-X. View

11.

Arnold D, Taylor D, Radda G . Investigation of human mitochondrial myopathies by phosphorus magnetic resonance spectroscopy. Ann Neurol. 1985; 18(2):189-96. DOI: 10.1002/ana.410180205. View

12.

Challiss R, Hayes D, Radda G . A 31P-NMR study of the acute effects of altered beta-adrenoceptor stimulation on the bioenergetics of skeletal muscle during contraction. Biochem Pharmacol. 1988; 37(24):4653-9. DOI: 10.1016/0006-2952(88)90334-6. View

13.

Scheuermann-Freestone M, Madsen P, Manners D, Blamire A, Buckingham R, Styles P . Abnormal cardiac and skeletal muscle energy metabolism in patients with type 2 diabetes. Circulation. 2003; 107(24):3040-6. DOI: 10.1161/01.CIR.0000072789.89096.10. View

14.

Radda G, Kemp G, Styles P, Taylor D . Control of oxidative phosphorylation in muscle. Biochem Soc Trans. 1993; 21 ( Pt 3)(3):762-4. DOI: 10.1042/bst0210762. View

15.

Kemp G, Thompson C, Barnes P, Radda G . Comparisons of ATP turnover in human muscle during ischemic and aerobic exercise using 31P magnetic resonance spectroscopy. Magn Reson Med. 1994; 31(3):248-58. DOI: 10.1002/mrm.1910310303. View

16.

van Loon L, Goodpaster B . Increased intramuscular lipid storage in the insulin-resistant and endurance-trained state. Pflugers Arch. 2005; 451(5):606-16. DOI: 10.1007/s00424-005-1509-0. View

17.

Kemp G, Radda G . Quantitative interpretation of bioenergetic data from 31P and 1H magnetic resonance spectroscopic studies of skeletal muscle: an analytical review. Magn Reson Q. 1994; 10(1):43-63. View

18.

RASMUSSEN U, Rasmussen H, Krustrup P, Quistorff B, Saltin B, Bangsbo J . Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab. 2001; 280(2):E301-7. DOI: 10.1152/ajpendo.2001.280.2.E301. View

19.

Argov Z, De Stefano N, Taivassalo T, Chen J, Karpati G, Arnold D . Abnormal oxidative metabolism in exercise intolerance of undetermined origin. Neuromuscul Disord. 1997; 7(2):99-104. DOI: 10.1016/s0960-8966(97)00426-4. View

20.

Schunk K, Pitton M, Duber C, Kersjes W, Schadmand-Fischer S, Thelen M . Dynamic phosphorus-31 magnetic resonance spectroscopy of the quadriceps muscle: effects of age and sex on spectroscopic results. Invest Radiol. 1999; 34(2):116-25. DOI: 10.1097/00004424-199902000-00004. View