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Retrograde Neuronal Tracing with a Deletion-mutant Rabies Virus

Overview
Journal Nat Methods
Specialties Biomedical Engineering
Pathology
Date 2006 Dec 21
PMID 17179932
Citations 379
Authors
Ian R Wickersham
Stefan Finke
Karl-Klaus Conzelmann
Edward M Callaway
Affiliations
Soon will be listed here.
Abstract

We have constructed a deletion-mutant rabies virus encoding EGFP and find it to be an excellent tool for studying detailed morphology and physiology of neurons projecting to injection sites within the mammalian brain. The virus cannot spread beyond initially infected cells yet, unlike other viral vectors, replicates its core within them. The cells therefore fluoresce intensely, revealing fine dendritic and axonal structure with no background from partially or faintly labeled cells.

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