The Cps Gene Cluster of Salmonella Strain LT2 Includes a Second Mannose Pathway: Sequence of Two Genes and Relationship to Genes in the Rfb Gene Cluster

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Jun 1

PMID 1712067

Citations 25

Authors

G Stevenson

S J Lee

L K Romana

P R REEVES

Affiliations

Soon will be listed here.

Abstract

We report the presence in Salmonella enterica strain LT2 (serovar thyphimurium) of duplicate genes for two steps in the synthesis of GDP-mannose. The previously known genes, rfbK (phosphomannomutase) and rfbM (mannose-1-phosphate guanyltransferase), are part of the gene cluster for the O antigen. The two new genes, cpsB and cpsG, respectively, are thought to be part of the gene cluster for the M antigen capsular polysaccharide, present in many Enterobacteriaceae. The two genes have been sequenced and have a GC content of 0.61, suggesting an origin outside of Salmonella. Comparison of the inferred protein sequences for cpsB and rfbM shows 57% identity of amino acids whereas for cpsG and rfbK there is only 19% identity. It is suggested that the greater divergence between cpsG and rfbK may be due to a period of accelerated evolution, perhaps precipitated by transfer of the genes from another species.

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