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Altered Deoxyribonucleotide Pools in P2 Eductants of Escherichia Coli K-12 Due to Deletion of the Dcd Gene

Overview
Journal J Bacteriol
Specialty Microbiology
Date 1976 May 1
PMID 177407
Citations 62
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Abstract

Deletion of the Escherichia coli K-12 chromosome associated with P2 mediated education extend through the structural gene for uridine kinase, udk, and the dcd gene encoding 2'-deoxycytidine 5'-triphosphate deaminase. The lack of uridine kinase makes a positive selection possible for these strains. Due to the dcd mutation, P2 eductants show large alterations in their deoxyribonucleoside triphosphate pools.

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References
1.
LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

2.
Beck C, Eisenhardt A, NEUHARD J . Deoxycytidine triphosphate deaminase of Salmonella typhimurium. Purification and characterization. J Biol Chem. 1975; 250(2):609-16. View

3.
Beck C, Ingraham J, NEUHARD J, Thomassen E . Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium. J Bacteriol. 1972; 110(1):219-28. PMC: 247401. DOI: 10.1128/jb.110.1.219-228.1972. View

4.
MUNCH-PETERSEN A . Mutants of Escherichia coli unable to metabolize cytidine: isolation and characterization. Mol Gen Genet. 1973; 126(2):177-86. DOI: 10.1007/BF00330992. View

5.
Beck C, Ingraham J, NEUHARD J . Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. II. Uridine kinase, cytosine deaminase and thymidine kinase. Mol Gen Genet. 1972; 115(3):208-15. DOI: 10.1007/BF00268884. View