Protein Encoded by HSV-1 Stimulation-related Gene 1 (HSRG1) Interacts with and Inhibits SV40 Large T Antigen

Overview

Journal Cell Prolif

Date 2006 Nov 18

PMID 17109635

Citations 2

Authors

H X Guo

W Cun

L D Liu

S Z Dong

L C Wang

C H Dong

Q H Li

Affiliations

Soon will be listed here.

Abstract

Herpes simplex virus (HSV)-1 stimulation-related gene 1 (HSRG1) protein expression is induced in HSV-1 infected cells. We found that HSRG1 interacts with SV40 large T antigen (LT) in yeast two-hybrid assay and bimolecular fluorescence complementation (BiFC) assay. This interaction alters LT's regulation of the SV40 promoter and its ability to influence the cell cycle. Choramphenicol acetyl-transferase (CAT) assays revealed that initiation of gene transcription by LT is changed by HSRG1 expression. HSRG1 inhibits the ability of LT to activate SV40 late gene transcription. Further data indicate that the ability of LT protein to stimulate S-phase entry is also inhibited by the expression of HSRG1. The results of a colony-forming assay suggested that expression of HSRG1 in cells transfected by LT gene decreased the rate of colony formation. Yeast two-hybrid beta-galactosidase assay revealed that amino acid residues 132-450 in LT bind HSRG1.

Citing Articles

Dynamic Detection of the E3-PROTAC-Target Protein Ternary Complex and Bimolecular Fluorescence Complementation.

Lei K, Sheng Y, Li Y, Zhou Z, Zhu X, Huang K ACS Omega. 2024; 9(50):49739-49748.

PMID: 39713624 PMC: 11656243. DOI: 10.1021/acsomega.4c08186.

Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells.

Kerppola T Annu Rev Biophys. 2008; 37:465-87.

PMID: 18573091 PMC: 2829326. DOI: 10.1146/annurev.biophys.37.032807.125842.

References

Ott R, Rehfuess C, Podust V, Clark J, Fanning E . Role of the p68 subunit of human DNA polymerase alpha-primase in simian virus 40 DNA replication. Mol Cell Biol. 2002; 22(16):5669-78. PMC: 133971. DOI: 10.1128/MCB.22.16.5669-5678.2002. View

Ullrich C, Groopman J, Ganju R . HIV-1 gp120- and gp160-induced apoptosis in cultured endothelial cells is mediated by caspases. Blood. 2000; 96(4):1438-42. View

Alwine J, Picardi J . Activity of simian virus 40 late promoter elements in the absence of large T antigen: evidence for repression of late gene expression. J Virol. 1986; 60(2):400-4. PMC: 288906. DOI: 10.1128/JVI.60.2.400-404.1986. View

Khoury G, May E . Regulation of early and late simian virus 40 transcription: overproduction of early viral RNA in the absence of a functional T-antigen. J Virol. 1977; 23(1):167-76. PMC: 515812. DOI: 10.1128/JVI.23.1.167-176.1977. View

Zhai W, Tuan J, Comai L . SV40 large T antigen binds to the TBP-TAF(I) complex SL1 and coactivates ribosomal RNA transcription. Genes Dev. 1997; 11(12):1605-17. DOI: 10.1101/gad.11.12.1605. View

Hu C, Kerppola T . Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol. 2003; 21(5):539-45. PMC: 1820765. DOI: 10.1038/nbt816. View

Li Q, Zhao H, Jiang L, Che Y, Dong C, Wang L . An SR-protein induced by HSVI binding to cells functioning as a splicing inhibitor of viral pre-mRNA. J Mol Biol. 2002; 316(4):887-94. DOI: 10.1006/jmbi.2001.5318. View

Hansen U, Tenen D, Livingston D, Sharp P . T antigen repression of SV40 early transcription from two promoters. Cell. 1981; 27(3 Pt 2):603-13. DOI: 10.1016/0092-8674(81)90402-5. View

Wiekowski M, Schwarz M, Stahl H . Simian virus 40 large T antigen DNA helicase. Characterization of the ATPase-dependent DNA unwinding activity and its substrate requirements. J Biol Chem. 1988; 263(1):436-42. View

10.

Scholle F, Li K, Bodola F, Ikeda M, Luxon B, Lemon S . Virus-host cell interactions during hepatitis C virus RNA replication: impact of polyprotein expression on the cellular transcriptome and cell cycle association with viral RNA synthesis. J Virol. 2004; 78(3):1513-24. PMC: 321413. DOI: 10.1128/jvi.78.3.1513-1524.2004. View

11.

Ray N, Enquist L . Transcriptional response of a common permissive cell type to infection by two diverse alphaherpesviruses. J Virol. 2004; 78(7):3489-501. PMC: 371087. DOI: 10.1128/jvi.78.7.3489-3501.2004. View

12.

Sullivan C, Pipas J . T antigens of simian virus 40: molecular chaperones for viral replication and tumorigenesis. Microbiol Mol Biol Rev. 2002; 66(2):179-202. PMC: 120785. DOI: 10.1128/MMBR.66.2.179-202.2002. View

13.

Stubdal H, Zalvide J, DeCaprio J . Simian virus 40 large T antigen alters the phosphorylation state of the RB-related proteins p130 and p107. J Virol. 1996; 70(5):2781-8. PMC: 190135. DOI: 10.1128/JVI.70.5.2781-2788.1996. View

14.

Johnston S, Yu X, Mertz J . The major transcriptional transactivation domain of simian virus 40 large T antigen associates nonconcurrently with multiple components of the transcriptional preinitiation complex. J Virol. 1996; 70(2):1191-202. PMC: 189928. DOI: 10.1128/JVI.70.2.1191-1202.1996. View

15.

Reed S, Ferguson J, Davis R, Stark G . T antigen binds to simian virus 40 DNA at the origin of replication. Proc Natl Acad Sci U S A. 1975; 72(4):1605-9. PMC: 432587. DOI: 10.1073/pnas.72.4.1605. View

16.

Tegtmeyer P . Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972; 10(4):591-8. PMC: 356508. DOI: 10.1128/JVI.10.4.591-598.1972. View

17.

Butel J, Lednicky J . Cell and molecular biology of simian virus 40: implications for human infections and disease. J Natl Cancer Inst. 1999; 91(2):119-34. DOI: 10.1093/jnci/91.2.119. View

18.

Nishigaki K, Hanson C, Ohashi T, Thompson D, Muszynski K, Ruscetti S . Erythroid cells rendered erythropoietin independent by infection with Friend spleen focus-forming virus show constitutive activation of phosphatidylinositol 3-kinase and Akt kinase: involvement of insulin receptor substrate-related adapter proteins. J Virol. 2000; 74(7):3037-45. PMC: 111802. DOI: 10.1128/jvi.74.7.3037-3045.2000. View

19.

Damania B, Mital R, Alwine J . Simian virus 40 large T antigen interacts with human TFIIB-related factor and small nuclear RNA-activating protein complex for transcriptional activation of TATA-containing polymerase III promoters. Mol Cell Biol. 1998; 18(3):1331-8. PMC: 108846. DOI: 10.1128/MCB.18.3.1331. View

20.

Sladek T, Laffin J, Lehman J, Jacobberger J . A subset of cells expressing SV40 large T antigen contain elevated p53 levels and have an altered cell cycle phenotype. Cell Prolif. 2000; 33(2):115-25. PMC: 6496574. DOI: 10.1046/j.1365-2184.2000.00168.x. View