Total Protein Extraction with TCA-acetone

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2006 Nov 10

PMID 17093296

Citations 69

Authors

Valerie Mechin

Catherine Damerval

Michel Zivy

Affiliations

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Abstract

We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneous precipitation and denaturation with TCA and 2ME in cold acetone. We also describe protein solubilization prior to IEF, either in classical rod gels or in IPGs, using two different solutions. The procedure is easy to carry out. The major caveats are (1) keep samples at low temperature during extraction, and then (2) manage protein samples at about 22 to 25 degrees C to avoid urea precipitation.

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