DDB1 Functions As a Linker to Recruit Receptor WD40 Proteins to CUL4-ROC1 Ubiquitin Ligases

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2006 Nov 3

PMID 17079684

Citations 199

Authors

Yizhou Joseph He

Chad M McCall

Jian Hu

Yaxue Zeng

Yue Xiong

Affiliations

Soon will be listed here.

Abstract

Cullins assemble the largest family of ubiquitin ligases by binding with ROC1 and various substrate receptors. CUL4 function is linked with many cellular processes, but its substrate-recruiting mechanism remains elusive. We identified a protein motif, the DWD box (DDB1-binding WD40 protein), and demonstrated the binding of 15 DWD proteins with DDB1-CUL4A. We provide evidence supporting the critical function of the DWD box and DDB1's role as the linker mediating DWD protein association with CUL4A. A database search predicts that about one-third of WD40 proteins, 90 in humans, contain the DWD box, suggesting a potentially large number of DWD-DDB1-CUL4-ROC1 E3 ligases.

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