Unexpected High Polymorphism at the FABP4 Gene Unveils a Complex History for Pig Populations

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2006 Oct 24

PMID 17057239

Citations 15

Authors

Ana Ojeda

Julio Rozas

Josep M Folch

Miguel Perez-Enciso

Affiliations

Soon will be listed here.

Abstract

Fatty acid bing protein 4 (FABP4) plays a key role in fat regulation in mammals and is a strong positional candidate gene for the FAT1 quantitative trait locus located on porcine chromosome 4. DNA resequencing of the FABP4 gene region in 23 pigs from 10 breeds and wild boar revealed 134 variants in 6.4 kb, representing a silent nucleotide diversity of piS=0.01, much higher than reported so far in animal domestic species. Moreover, this diversity was highly structured. Also strikingly, the FABP4 phylogenetic tree did not show any geographical or breed origin clustering, with distant breeds sharing similar haplotypes and some of the most heterozygous samples pertaining to highly inbred animals like Iberian Guadyerbas (inbreeding coefficient approximately 0.3) or British Tamworth. In contrast, the cytochrome b (mtDNA) phylogenetic tree was coherent with geographical origin. The estimated age of the most recent common ancestor for the most divergent Iberian or Tamworth haplotypes was much older than domestication. An additional panel of 100 pigs from 8 different breeds and wild boar from Spain, Tunisia, Sardinia, and Japan was genotyped for seven selected single nucleotide polymorphisms and shows that high variability at the porcine FABP4 is the rule rather than the exception. Pig populations, even highly inbred, can maintain high levels of variability for surprisingly long periods of time.

Citing Articles

Expression analysis of candidate genes for fatty acid composition in adipose tissue and identification of regulatory regions.

Revilla M, Puig-Oliveras A, Crespo-Piazuelo D, Criado-Mesas L, Castello A, Fernandez A Sci Rep. 2018; 8(1):2045.

PMID: 29391556 PMC: 5794915. DOI: 10.1038/s41598-018-20473-3.

Contacts in the last 90,000 years over the Strait of Gibraltar evidenced by genetic analysis of wild boar (Sus scrofa).

Soria-Boix C, Donat-Torres M, Urios V PLoS One. 2017; 12(7):e0181929.

PMID: 28742834 PMC: 5526546. DOI: 10.1371/journal.pone.0181929.

Haplotypic diversity of porcine LEP and LEPR genes involved in growth and fatness regulation.

Perez-Montarelo D, Rodriguez M, Fernandez A, Benitez R, Garcia F, Silio L J Appl Genet. 2015; 56(4):525-533.

PMID: 25910874 DOI: 10.1007/s13353-015-0284-7.

Identification of more than two paternal haplotypes of the ovine fatty acid-binding protein 4 (FABP4) gene in half-sib families: evidence of intragenic meiotic recombination.

Yan W, Zhou H, Luo Y, Hu J, Hickford J PLoS One. 2014; 9(2):e88691.

PMID: 24523930 PMC: 3921221. DOI: 10.1371/journal.pone.0088691.

Allelic variation in ovine fatty acid-binding protein (FABP4) gene.

Yan W, Zhou H, Luo Y, Hu J, Hickford J Mol Biol Rep. 2012; 39(12):10621-5.

PMID: 23086266 DOI: 10.1007/s11033-012-1951-y.

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