Mapping RNase T1-resistant Oligonucleotides of Avian Tumor Virus RNAs: Sarcoma-specific Oligonucleotides Are Near the Poly(A) End and Oligonucleotides Common to Sarcoma and Transformation-defective Viruses Are at the Poly(A) End

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1975 Oct 1

PMID 170411

Citations 108

Authors

L H Wang

P Duesberg

K Beemon

P K Vogt

Affiliations

Soon will be listed here.

Abstract

The large RNase T1-resistant oligonucleotides of the nondefective (nd) Rous sarcoma virus (RSV): Prague RSV of subgroup B (PR-B), PR-C and B77 of subgroup C; of their transformation-defective (td0 deletion mutants: td PR-B, td PR-C, and td B77; and of replication-defective (rd) RSV(-) were completely or partially mapped on the 30 to 40S viral RNAs. The location of a given oligonucleotide relative to the poly(A) terminus of the viral RNAs was directly deduced from the smallest size of the poly(A)-tagged RNA fragment from which it could be isolated. Identification of distinct oligonucleotides was based on their location in the electrophoretic/chromatographic fingerprint pattern and on analysis of their RNase A-resistant fragments. The following results were obtained. (i) The number of large oligonucleotides per poly(A)-tagged ffagment increased with increasing size of the fragment. This implies that the genetic map is linear and that a given RNase T1-resistant oligonucleotides has, relative to the poly(A) end, the same location on all 30 to 40S RNA subunits of a given 60 to 70S viral RNA complex, (ii) Three sarcoma-specific oligonucleotides were identified in the RNAs of Pr-B, PR-C and B77 by comparison with the RNAs of the corresponding td viruses...

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