Differential Stimulation of Murine Lymphoma Growth in Vitro by Normal and BCG-activated Macrophages

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1975 Oct 1

PMID 170358

Citations 25

Authors

C F Nathan

W D TERRY

Affiliations

Soon will be listed here.

Abstract

Peritoneal macrophages from mice infected with Bacille Calmette-Guérin (BCG) and from normal mice were examined for their effects in vitro on thymidine uptake by 10 murine lymphomas, a murine fibroblast line, and a guinea pig hepatoma. Only the murine fibroblast line showed growth inhibition in the presence of BCG macrophages. For the majority of tumors, normal macrophages were profoundly stimulatory to tumor cell DNA synthesis, while BCG macrophages were much less stimulatory, without being frankly inhibitory. The effect of 2-mercaptoethanol on tumor cell growth was also studied. All lymphomas stimulated to grow more rapidly in vitro by normal macrophages were stimulated to a similar degree by 2-mercaptoethanol.

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References

Calderon J, UNANUE E . Two biological activities regulating cell proliferation found in cultures of peritoneal exudate cells. Nature. 1975; 253(5490):359-61. DOI: 10.1038/253359a0. View

AMIEL J . [Non specific active immunotherapy by B.C.G. in virus induced leukemia in E male G2 mice in isogenic receiptients]. Rev Fr Etud Clin Biol. 1967; 12(9):912-4. View

Rocklin R, Winston C, David J . Activation of human blood monocytes by products of sensitized lymphocytes. J Clin Invest. 1974; 53(2):559-64. PMC: 301499. DOI: 10.1172/JCI107590. View

Gallily R . Allogeneic recognition and killing capacity of immune macrophages in mixed macrophage cultures (MMC). Cell Immunol. 1975; 15(2):419-31. DOI: 10.1016/0008-8749(75)90019-2. View

Dimitriu A, Dy M, Thomson N, Hamburger J . Macrophage cytotoxicity in the mouse immune response against a skin allograft. J Immunol. 1975; 114(1 Pt 1):195-9. View

Kirchner H, Muchmore A, Chused T, HOLDEN H, Herberman R . Inhibition of proliferation of lymphoma cells and T lymphocytes by suppressor cells from spleens of tumor-bearing mice. J Immunol. 1975; 114(1 Pt 1):206-10. View

Keller R . Major changes in lymphocyte proliferation evoked by activated macrophages. Cell Immunol. 1975; 17(2):542-51. DOI: 10.1016/s0008-8749(75)80058-x. View

KISSANE J, Robins E . The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem. 1958; 233(1):184-8. View

Lemonde P, Clode M . Effect of BCG infection on leukemia and polyoma in mice and hamsters. Proc Soc Exp Biol Med. 1962; 111:739-42. DOI: 10.3181/00379727-111-27908. View

10.

GRANGER G, WEISER R . HOMOGRAFT TARGET CELLS: SPECIFIC DESTRUCTION IN VITRO BY CONTACT INTERACTION WITH IMMUNE MACROPHAGES. Science. 1964; 145(3639):1427-9. DOI: 10.1126/science.145.3639.1427. View

11.

Piessens W, Churchill Jr W, David . Macrophages activated in vitro with lymphocyte mediators kill neoplastic but not normal cells. J Immunol. 1975; 114(1 Pt 2):293-9. View

12.

Hibbs Jr J, Lambert Jr L, Remington J . Possible role of macrophage mediated nonspecific cytotoxicity in tumour resistance. Nat New Biol. 1972; 235(54):48-50. DOI: 10.1038/newbio235048a0. View

13.

Shevach E, Stobo J, Green I . Immunoglobulin and theta-bearing murine leukemias and lymphomas. J Immunol. 1972; 108(5):1146-51. View

14.

Nathan C, Remold H, David J . Characterization of a lymphocyte factor which alters macrophage functions. J Exp Med. 1973; 137(2):275-90. PMC: 2139484. DOI: 10.1084/jem.137.2.275. View

15.

Lohmann-Matthes M, Ziegler F, Fischer H . Macrophage cytotoxicity factor. A product of in vitro sensitized thymus-dependent cells. Eur J Immunol. 1973; 3(1):56-8. DOI: 10.1002/eji.1830030112. View

16.

Krahenbuhl J, Remington J . The role of activated macrophages in specific and nonspecific cytostasis of tumor cells. J Immunol. 1974; 113(2):507-16. View

17.

MATHE G, Pouillart P, LAPEYRAQUE F . Active immunotherapy of L1210 leukaemia applied after the graft of tumour cells. Br J Cancer. 1969; 23(4):814-24. PMC: 2008494. DOI: 10.1038/bjc.1969.101. View

18.

Shevach E, Herberman R, Lieberman R, Frank M, Green I . Receptors for immunoglobulin and complement on mouse leukemias and lymphomas. J Immunol. 1972; 108(2):325-8. View

19.

Namba Y, Hanaoka M . Immunocytology of cultured IgM-forming cells of mouse. I. Requirement of phagocytic cell factor for the growth of IgM-forming tumor cells in tissue culture. J Immunol. 1972; 109(6):1193-200. View

20.

Zembala M, Ptak W, Hanczakowska M . The role of macrophages in the cytotoxic killing of tumour cells in vitro. I. Primary immunization of lymphocytes in vitro for target cell killing and the mechanism of lymphocyte-macrophage cooperation. Immunology. 1973; 25(4):631-44. PMC: 1423159. View