Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2006 Oct 13

PMID 17035495

Citations 30

Authors

Isabel Jado

Raquel Escudero

Horacio Gil

Maria Isabel Jimenez-Alonso

Rita Sousa

Ana L Garcia-Perez

Manuela Rodriguez-Vargas

Bruno Lobo

Pedro Anda

Affiliations

Soon will be listed here.

Abstract

We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.

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