Incidence of Tick-borne Spotted Fever Group Rickettsia Species in Rodents in Two Regions in Kazakhstan

Overview

Journal Sci Rep

Specialty Science

Date 2022 Sep 1

PMID 36050456

Authors

E Wagner

N Tukhanova

A Shin

N Turebekov

Z Shapiyeva

A Shevtsov

T Nurmakhanov

V Sutyagin

A Berdibekov

N Maikanov

I Lezdinsh

K Freimuller

R Ehmann

C Ehrhardt

S Essbauer

L Peintner

Affiliations

Soon will be listed here.

Abstract

Records on the distribution of Rickettsia spp. in their natural hosts in Central Asia are incomplete. Rodents and small mammals are potential natural reservoirs for Rickettsiae in their natural lifecycle. Studies about the maintenance of Rickettsia in wild animals are available for Western nations, but-to our knowledge-no studies and data are available in the Republic of Kazakhstan so far. The first case description of Rickettsioses in Kazakhstan was made in the 1950ies in the Almaty region and now Kyzylorda, East Kazakhstan, Pavlodar and North Kazakhstan are endemic areas. The existence of murine and endemic typhus was proven in arthropod vectors in the regions Kyzylorda and Almaty. Here we show for the first time investigations on tick-borne Rickettsia species detected by a pan-rickettsial citrate synthase gene (gltA) real-time PCR in ear lobes of small mammals (n = 624) in Kazakhstan. From all analysed small mammals 2.72% were positive for Rickettsia raoultii, R. slovaca or R. conorii. Sequencing of the rickettsial gene OmpAIV and the 23S-5S interspacer region revealed a similar heritage of identified Rickettsia species that was observed in ticks in previous studies from the region. In summary, this study proves that rodents in Kazakhstan serve as a natural reservoir of Rickettsia spp.

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References

Tukhanova N, Shin A, Turebekov N, Nurmakhanov T, Abdiyeva K, Shevtsov A . Molecular Characterisation and Phylogeny of Tula Virus in Kazakhstan. Viruses. 2022; 14(6). PMC: 9230364. DOI: 10.3390/v14061258. View

Parola P, Paddock C, Socolovschi C, Labruna M, Mediannikov O, Kernif T . Update on tick-borne rickettsioses around the world: a geographic approach. Clin Microbiol Rev. 2013; 26(4):657-702. PMC: 3811236. DOI: 10.1128/CMR.00032-13. View

El Karkouri K, Ghigo E, Raoult D, Fournier P . Genomic evolution and adaptation of arthropod-associated Rickettsia. Sci Rep. 2022; 12(1):3807. PMC: 8907221. DOI: 10.1038/s41598-022-07725-z. View

Legendre K, Macaluso K . Rickettsia felis: A Review of Transmission Mechanisms of an Emerging Pathogen. Trop Med Infect Dis. 2018; 2(4). PMC: 6082062. DOI: 10.3390/tropicalmed2040064. View

Fournier P, Raoult D . Current knowledge on phylogeny and taxonomy of Rickettsia spp. Ann N Y Acad Sci. 2009; 1166:1-11. DOI: 10.1111/j.1749-6632.2009.04528.x. View

Tomassone L, Berriatua E, de Sousa R, Duscher G, Mihalca A, Silaghi C . Neglected vector-borne zoonoses in Europe: Into the wild. Vet Parasitol. 2018; 251:17-26. DOI: 10.1016/j.vetpar.2017.12.018. View

Fournier P, Roux V, Raoult D . Phylogenetic analysis of spotted fever group rickettsiae by study of the outer surface protein rOmpA. Int J Syst Bacteriol. 1998; 48 Pt 3:839-49. DOI: 10.1099/00207713-48-3-839. View

Robinson M, Satjanadumrong J, Hughes T, Stenos J, Blacksell S . Diagnosis of spotted fever group infections: the Asian perspective. Epidemiol Infect. 2019; 147:e286. PMC: 6805790. DOI: 10.1017/S0950268819001390. View

Guo L, Mu L, Xu J, Jiang S, Wang A, Chen C . Rickettsia raoultii in Haemaphysalis erinacei from marbled polecats, China-Kazakhstan border. Parasit Vectors. 2015; 8:461. PMC: 4573940. DOI: 10.1186/s13071-015-1065-1. View

10.

Hay J, Yeh K, Dasgupta D, Shapieva Z, Omasheva G, Deryabin P . Biosurveillance in Central Asia: Successes and Challenges of Tick-Borne Disease Research in Kazakhstan and Kyrgyzstan. Front Public Health. 2016; 4:4. PMC: 4740946. DOI: 10.3389/fpubh.2016.00004. View

11.

rehacek J, Zupancicova M, Kovacova E, Urvolgyi J, BREZINA R . Study of rickettsioses in Slovakia. III. Experimental infection of Apodemus flavicollis Melch. by rickettsiae of the spotted fever (SF) group isolated in Slovakia. J Hyg Epidemiol Microbiol Immunol. 1976; 21(3):306-13. View

12.

Stanczak J, Racewicz M, Michalik J, Cieniuch S, Sikora B, Skoracki M . Prevalence of infection with Rickettsia helvetica in feeding ticks and their hosts in western Poland. Clin Microbiol Infect. 2009; 15 Suppl 2:328-9. DOI: 10.1111/j.1469-0691.2008.02261.x. View

13.

Turebekov N, Abdiyeva K, Yegemberdiyeva R, Kuznetsov A, Dmitrovskiy A, Yeraliyeva L . Occurrence of Anti-Rickettsia spp. Antibodies in Hospitalized Patients with Undifferentiated Febrile Illness in the Southern Region of Kazakhstan. Am J Trop Med Hyg. 2021; 104(6):2000-2008. PMC: 8176513. DOI: 10.4269/ajtmh.20-0388. View

14.

Gajda E, Hildebrand J, Sprong H, Bunkowska-Gawlik K, Perec-Matysiak A, Coipan E . Spotted fever rickettsiae in wild-living rodents from south-western Poland. Parasit Vectors. 2017; 10(1):413. PMC: 5585920. DOI: 10.1186/s13071-017-2356-5. View

15.

rehacek J, Urvolgyi J, Kocianova E, Jedlicka L . Susceptibility of some species of rodents to rickettsiae. Folia Parasitol (Praha). 1992; 39(3):265-84. View

16.

Shpynov S, Parola P, Rudakov N, Samoilenko I, Tankibaev M, Tarasevich I . Detection and identification of spotted fever group rickettsiae in Dermacentor ticks from Russia and central Kazakhstan. Eur J Clin Microbiol Infect Dis. 2002; 20(12):903-5. DOI: 10.1007/s10096-001-0638-4. View

17.

Vallee J, Thaojaikong T, Moore C, Phetsouvanh R, Richards A, Souris M . Contrasting spatial distribution and risk factors for past infection with scrub typhus and murine typhus in Vientiane City, Lao PDR. PLoS Negl Trop Dis. 2010; 4(12):e909. PMC: 2998433. DOI: 10.1371/journal.pntd.0000909. View

18.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

19.

Turebekov N, Abdiyeva K, Yegemberdiyeva R, Dmitrovsky A, Yeraliyeva L, Shapiyeva Z . Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan. Parasit Vectors. 2019; 12(1):197. PMC: 6500025. DOI: 10.1186/s13071-019-3440-9. View

20.

Shpynov S, Fournier P, Rudakov N, Tankibaev M, Tarasevich I, Raoult D . Detection of a rickettsia closely related to Rickettsia aeschlimannii, "Rickettsia heilongjiangensis," Rickettsia sp. strain RpA4, and Ehrlichia muris in ticks collected in Russia and Kazakhstan. J Clin Microbiol. 2004; 42(5):2221-3. PMC: 404608. DOI: 10.1128/JCM.42.5.2221-2223.2004. View