Signature-tagged Transposon Mutagenesis Identifies Novel Mycobacterium Tuberculosis Genes Involved in the Parasitism of Human Macrophages

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2006 Oct 13

PMID 17030567

Citations 40

Authors

Vania Rosas-Magallanes

Gustavo Stadthagen-Gomez

Jean Rauzier

Luis B Barreiro

Ludovic Tailleux

Frederic Boudou

Ruth Griffin

Jerome Nigou

Mary Jackson

Brigitte Gicquel

Olivier Neyrolles

Affiliations

Soon will be listed here.

Abstract

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

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