A Potential Protein-RNA Recognition Event Along the RISC-loading Pathway from the Structure of A. Aeolicus Argonaute with Externally Bound SiRNA

Overview

Journal Structure

Publisher Cell Press

Specialties Biochemistry
Biotechnology
Cell Biology
Molecular Biology

Date 2006 Oct 10

PMID 17027504

Citations 18

Authors

Yu-Ren Yuan

Yi Pei

Hong-Ying Chen

Thomas Tuschl

Dinshaw J Patel

Affiliations

Soon will be listed here.

Abstract

Argonaute proteins are key components of the RNA-induced silencing complex (RISC). They provide both architectural and catalytic functionalities associated with small interfering RNA (siRNA) guide strand recognition and subsequent guide strand-mediated cleavage of complementary mRNAs. We report on the 3.0 A crystal structures of 22-mer and 26-mer siRNAs bound to Aquifex aeolicus Argonaute (Aa-Ago), where one 2 nt 3' overhang of the siRNA inserts into a cavity positioned on the outer surface of the PAZ-containing lobe of the bilobal Aa-Ago architecture. The first overhang nucleotide stacks over a tyrosine ring, while the second overhang nucleotide, together with the intervening sugar-phosphate backbone, inserts into a preformed surface cavity. Photochemical crosslinking studies on Aa-Ago with 5-iodoU-labeled single-stranded siRNA and siRNA duplex provide support for this externally bound siRNA-Aa-Ago complex. The structure and biochemical data together provide insights into a protein-RNA recognition event potentially associated with the RISC-loading pathway.

Citing Articles

Argonaute protein-based nucleic acid detection technology.

Wu Z, Yu L, Shi W, Ma J Front Microbiol. 2023; 14:1255716.

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Prokaryotic Argonaute Proteins as a Tool for Biotechnology.

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The Expanded Universe of Prokaryotic Argonaute Proteins.

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PMID: 30563906 PMC: 6299218. DOI: 10.1128/mBio.01935-18.

DNA interference and beyond: structure and functions of prokaryotic Argonaute proteins.

Lisitskaya L, Aravin A, Kulbachinskiy A Nat Commun. 2018; 9(1):5165.

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Improved siRNA delivery efficiency via solvent-induced condensation of micellar nanoparticles.

Wu J, Qu W, Williford J, Ren Y, Jiang X, Jiang X Nanotechnology. 2017; 28(20):204002.

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