Chronotropic Response of Cultured Neonatal Rat Ventricular Myocytes to Short-term Fluid Shear

Overview

Journal Cell Biochem Biophys

Specialties Biochemistry
Biophysics
Cell Biology

Date 2006 Oct 3

PMID 17012753

Citations 16

Authors

Ilka Lorenzen-Schmidt

Geert W Schmid-Schonbein

Wayne R Giles

Andrew D McCulloch

Shu Chien

Jeffrey H Omens

Affiliations

Soon will be listed here.

Abstract

Ventricular myocytes are continuously exposed to fluid shear in vivo by relative movement of laminar sheets and adjacent cells. Preliminary observations have shown that neonatal myocytes respond to fluid shear by increasing their beating rate, which could have an arrhythmogenic effect under elevated shear conditions. The objective of this study is to investigate the characteristics of the fluid shear response in cultured myocytes and to study selected potential mechanisms. Cultured neonatal rat ventricular myocytes that were spontaneously beating were subjected to low shear rates (5-50/s) in a fluid flow chamber using standard culture medium. The beating rate was measured from digital microscopic recordings. The myocytes reacted to low shear rates by a graded and reversible increase in their spontaneous beating rate of up to 500%. The response to shear was substantially attenuated in the presence of the beta-adrenergic agonist isoproterenol (by 86+/-8%), as well as after incubation with integrin-blocking RGD peptides (by 92+/-8%). The results suggest that the beta-adrenergic signaling pathway and integrin activation, which are known to interact, may play an important role in the response mechanism.

Citing Articles

Remodeling of Ion Channel Trafficking and Cardiac Arrhythmias.

Blandin C, Gravez B, Hatem S, Balse E Cells. 2021; 10(9).

PMID: 34572065 PMC: 8468138. DOI: 10.3390/cells10092417.

Minimal contribution of IPR2 in cardiac differentiation and derived ventricular-like myocytes from human embryonic stem cells.

Zhang P, Huang J, Ou-Yang K, Liang H, Li M, Wang Y Acta Pharmacol Sin. 2020; 41(12):1576-1586.

PMID: 33037404 PMC: 7921119. DOI: 10.1038/s41401-020-00528-w.

Autoantibodies directed against α1-adrenergic receptor and endothelin receptor A in patients with prostate cancer.

Wallukat G, Jandrig B, Becker N, Wendler J, Gottel P, Muller J Auto Immun Highlights. 2020; 11(1):13.

PMID: 32977857 PMC: 7519497. DOI: 10.1186/s13317-020-00136-y.

The role of stretch, tachycardia and sodium-calcium exchanger in induction of early cardiac remodelling.

Djalinac N, Ljubojevic-Holzer S, Matzer I, Kolesnik E, Jandl K, Lohberger B J Cell Mol Med. 2020; 24(15):8732-8743.

PMID: 32573098 PMC: 7412684. DOI: 10.1111/jcmm.15504.

Fluid flow modulates electrical activity in cardiac hERG potassium channels.

Roy S, Mathew M J Biol Chem. 2018; 293(12):4289-4303.

PMID: 29305421 PMC: 5868271. DOI: 10.1074/jbc.RA117.000432.

References

Cerbai E, Pino R, Sartiani L, Mugelli A . Influence of postnatal-development on I(f) occurrence and properties in neonatal rat ventricular myocytes. Cardiovasc Res. 1999; 42(2):416-23. DOI: 10.1016/s0008-6363(99)00037-1. View

Dou J, Tseng W, Reese T, Wedeen V . Combined diffusion and strain MRI reveals structure and function of human myocardial laminar sheets in vivo. Magn Reson Med. 2003; 50(1):107-13. DOI: 10.1002/mrm.10482. View

Nauli S, Alenghat F, Luo Y, Williams E, Vassilev P, Li X . Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells. Nat Genet. 2003; 33(2):129-37. DOI: 10.1038/ng1076. View

Silva J, Rudy Y . Mechanism of pacemaking in I(K1)-downregulated myocytes. Circ Res. 2003; 92(3):261-3. PMC: 1890031. DOI: 10.1161/01.res.0000057996.20414.c6. View

Communal C, Singh M, Menon B, Xie Z, Colucci W, Singh K . beta1 integrins expression in adult rat ventricular myocytes and its role in the regulation of beta-adrenergic receptor-stimulated apoptosis. J Cell Biochem. 2003; 89(2):381-8. DOI: 10.1002/jcb.10520. View

Kong C, Bursac N, Tung L . Mechanoelectrical excitation by fluid jets in monolayers of cultured cardiac myocytes. J Appl Physiol (1985). 2005; 98(6):2328-36. DOI: 10.1152/japplphysiol.01084.2004. View

Torsoni A, Constancio S, Nadruz Jr W, Hanks S, Franchini K . Focal adhesion kinase is activated and mediates the early hypertrophic response to stretch in cardiac myocytes. Circ Res. 2003; 93(2):140-7. DOI: 10.1161/01.RES.0000081595.25297.1B. View

Paul S . Ventricular remodeling. Crit Care Nurs Clin North Am. 2004; 15(4):407-11. DOI: 10.1016/s0899-5885(02)00089-8. View

Janse M . Electrophysiological changes in heart failure and their relationship to arrhythmogenesis. Cardiovasc Res. 2004; 61(2):208-17. DOI: 10.1016/j.cardiores.2003.11.018. View

10.

Lakatta E . Beyond Bowditch: the convergence of cardiac chronotropy and inotropy. Cell Calcium. 2004; 35(6):629-42. DOI: 10.1016/j.ceca.2004.01.017. View

11.

Cheng Q, Ross R, Walsh K . Overexpression of the integrin beta(1A) subunit and the beta(1A) cytoplasmic domain modifies the beta-adrenergic regulation of the cardiac L-type Ca(2+)current. J Mol Cell Cardiol. 2004; 36(6):809-19. DOI: 10.1016/j.yjmcc.2004.03.006. View

12.

Coughlin M, Schmid-Schonbein G . Pseudopod projection and cell spreading of passive leukocytes in response to fluid shear stress. Biophys J. 2004; 87(3):2035-42. PMC: 1304606. DOI: 10.1529/biophysj.104.042192. View

13.

Dewey Jr C, Bussolari S, Gimbrone Jr M, Davies P . The dynamic response of vascular endothelial cells to fluid shear stress. J Biomech Eng. 1981; 103(3):177-85. DOI: 10.1115/1.3138276. View

14.

Reuter H, Cachelin A, de Peyer J, Kokubun S . Modulation of calcium channels in cultured cardiac cells by isoproterenol and 8-bromo-cAMP. Cold Spring Harb Symp Quant Biol. 1983; 48 Pt 1:193-200. DOI: 10.1101/sqb.1983.048.01.022. View

15.

Belval T, Hellums J, Solis R . The kinetics of platelet aggregation induced by fluid-shearing stress. Microvasc Res. 1984; 28(3):279-88. DOI: 10.1016/0026-2862(84)90001-3. View

16.

Reich K, Gay C, Frangos J . Fluid shear stress as a mediator of osteoblast cyclic adenosine monophosphate production. J Cell Physiol. 1990; 143(1):100-4. DOI: 10.1002/jcp.1041430113. View

17.

Sterpetti A, Cucina A, DAngelo L, Cardillo B, Cavallaro A . Response of arterial smooth muscle cells to laminar flow. J Cardiovasc Surg (Torino). 1992; 33(5):619-24. View

18.

Balligand J, Kelly R, Marsden P, Smith T, Michel T . Control of cardiac muscle cell function by an endogenous nitric oxide signaling system. Proc Natl Acad Sci U S A. 1993; 90(1):347-51. PMC: 45657. DOI: 10.1073/pnas.90.1.347. View

19.

Orita H, Fukasawa M, Hirooka S, Uchino H, Fukui K, Washio M . Modulation of cardiac myocyte beating rate and hypertrophy by cardiac fibroblasts isolated from neonatal rat ventricle. Jpn Circ J. 1993; 57(9):912-20. DOI: 10.1253/jcj.57.912. View

20.

Masuda H, Sperelakis N . Inwardly rectifying potassium current in rat fetal and neonatal ventricular cardiomyocytes. Am J Physiol. 1993; 265(4 Pt 2):H1107-11. DOI: 10.1152/ajpheart.1993.265.4.H1107. View