Asn- and Asp-mediated Interactions Between Transmembrane Helices During Translocon-mediated Membrane Protein Assembly

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2006 Sep 30

PMID 17008929

Citations 38

Authors

Nadja M Meindl-Beinker

Carolina Lundin

IngMarie Nilsson

Stephen H White

Gunnar von Heijne

Affiliations

Soon will be listed here.

Abstract

Inter-helix hydrogen bonding involving asparagine (Asn, N), glutamine (Gln, Q), aspartic acid (Asp, D) or glutamic acid (Glu, E) can drive efficient di- or trimerization of transmembrane helices in detergent micelles and lipid bilayers. Likewise, Asn-Asn and Asp-Asp pairs can promote the formation of helical hairpins during translocon-mediated membrane protein assembly in the endoplasmic reticulum. By in vitro translation of model integral membrane protein constructs in the presence of rough microsomes, we show that Asn- or Asp-mediated interactions with a neighbouring transmembrane helix can enhance the membrane insertion efficiency of a marginally hydrophobic transmembrane segment. Our observations suggest that inter-helix hydrogen bonds can form during Sec61 translocon-assisted insertion and thus could be important for membrane protein assembly.

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