Structure of the E. Coli Protein-conducting Channel Bound to a Translating Ribosome

Overview

Journal Nature

Specialty Science

Date 2005 Nov 18

PMID 16292303

Citations 93

Authors

Kakoli Mitra

Christiane Schaffitzel

Tanvir Shaikh

Florence Tama

Simon Jenni

Charles L Brooks 3rd

Nenad Ban

Joachim Frank

Affiliations

Soon will be listed here.

Abstract

Secreted and membrane proteins are translocated across or into cell membranes through a protein-conducting channel (PCC). Here we present a cryo-electron microscopy reconstruction of the Escherichia coli PCC, SecYEG, complexed with the ribosome and a nascent chain containing a signal anchor. This reconstruction shows a messenger RNA, three transfer RNAs, the nascent chain, and detailed features of both a translocating PCC and a second, non-translocating PCC bound to mRNA hairpins. The translocating PCC forms connections with ribosomal RNA hairpins on two sides and ribosomal proteins at the back, leaving a frontal opening. Normal mode-based flexible fitting of the archaeal SecYEbeta structure into the PCC electron microscopy densities favours a front-to-front arrangement of two SecYEG complexes in the PCC, and supports channel formation by the opening of two linked SecY halves during polypeptide translocation. On the basis of our observation in the translocating PCC of two segregated pores with different degrees of access to bulk lipid, we propose a model for co-translational protein translocation.

Citing Articles

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