Biochemical, Molecular, and Functional Characterization of PISCF-allatostatin, a Regulator of Juvenile Hormone Biosynthesis in the Mosquito Aedes Aegypti

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2006 Sep 14

PMID 16968697

Citations 23

Authors

Yiping Li

Salvador Hernandez-Martinez

Facundo Fernandez

Jaime G Mayoral

Pantelis Topalis

Horacio Priestap

Mario Perez

Arti Navare

Fernando G Noriega

Affiliations

Soon will be listed here.

Abstract

Aedes aegypti PISCF-allatostatin or allatostatin-C (Ae-AS-C) was isolated using a combination of high performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA). The matrix-assisted laser desorption/ionization time-of-flight (TOF) mass spectrum of positive ELISA fractions revealed a molecular mass of 1919.0 Da, in agreement with the sequence qIRYRQCYFNPISCF, with bridged cysteines. This sequence was confirmed by matrix-assisted laser desorption/ionization tandem TOF/TOF mass spectrometry analysis. The corresponding Ae-AS-C cDNA was amplified by PCR, and the sequence of the peptide was confirmed. An in vitro radiochemical assay was used to study the inhibitory effect of synthetic Ae-AS-C on juvenile hormone biosynthesis by the isolated corpora allata (CA) of adult female A. aegypti. The inhibitory action of synthetic Ae-AS-C was dose-dependent; with a maximum at 10(-9) m. Ae-AS-C showed no inhibitory activity in the presence of farnesoic acid, an immediate precursor of juvenile hormone, indicating that the Ae-AS-C target is located before the formation of farnesoic acid in the pathway. The sensitivity of the CA to inhibition by Ae-AS-C in the in vitro assay varied during the adult life; the CA was most sensitive during periods of low synthetic activity. In addition, the levels of Ae-AS-C in the brain were studied using ELISA and reached a maximum at 3 days after eclosion. These studies suggest that Ae-AS-C is an important regulator of CA activity in A. aegypti.

Citing Articles

Pharmacological Characterization of the Stick Insect Allatostatin-C Receptor with Its Endogenous Agonist.

Isbilir A, Sahbaz B, Tuncgenc G, Bunemann M, Lohse M, Birgul-Iyison N ACS Omega. 2020; 5(50):32183-32194.

PMID: 33376856 PMC: 7758886. DOI: 10.1021/acsomega.0c03382.

Characterization of G-protein coupled receptors from the blackback land crab Gecarcinus lateralis Y organ transcriptome over the molt cycle.

Tran N, Mykles D, Elizur A, Ventura T BMC Genomics. 2019; 20(1):74.

PMID: 30669976 PMC: 6341585. DOI: 10.1186/s12864-018-5363-9.

Mosquito Peptide Hormones: Diversity, Production, and Function.

Strand M, Brown M, Vogel K Adv In Insect Phys. 2019; 51:145-188.

PMID: 30662099 PMC: 6338476. DOI: 10.1016/bs.aiip.2016.05.003.

A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti.

Kang D, Barron M, Lovin D, Cunningham J, Eng M, Chadee D PLoS Negl Trop Dis. 2018; 12(6):e0006568.

PMID: 29889847 PMC: 6013235. DOI: 10.1371/journal.pntd.0006568.

A deep insight into the male and female sialotranscriptome of adult Culex tarsalis mosquitoes.

Ribeiro J, Martin-Martin I, Moreira F, Bernard K, Calvo E Insect Biochem Mol Biol. 2018; 95:1-9.

PMID: 29526772 PMC: 5927831. DOI: 10.1016/j.ibmb.2018.03.001.

References

Noriega F, Ribeiro J, Koener J, Valenzuela J, Hernandez-Martinez S, Pham V . Comparative genomics of insect juvenile hormone biosynthesis. Insect Biochem Mol Biol. 2006; 36(4):366-74. PMC: 2662747. DOI: 10.1016/j.ibmb.2006.01.013. View

Matthews H, Audsley N, Weaver R . Alanine substitution and deletion analogues of Manduca sexta allatostatin: structure-activity relationship on the spontaneous contractions of the foregut of larval Lacanobia oleracea. J Insect Physiol. 2005; 52(2):128-35. DOI: 10.1016/j.jinsphys.2005.07.006. View

Knudson D, Brown S, Severson D . Culicine genomics. Insect Biochem Mol Biol. 2002; 32(10):1193-7. DOI: 10.1016/s0965-1748(02)00082-6. View

Riehle M, Garczynski S, Crim J, Hill C, Brown M . Neuropeptides and peptide hormones in Anopheles gambiae. Science. 2002; 298(5591):172-5. DOI: 10.1126/science.1076827. View

Kreienkamp H, Larusson H, Witte I, Roeder T, Birgul N, Honck H . Functional annotation of two orphan G-protein-coupled receptors, Drostar1 and -2, from Drosophila melanogaster and their ligands by reverse pharmacology. J Biol Chem. 2002; 277(42):39937-43. DOI: 10.1074/jbc.M206931200. View

Bendena W, Donly B, Tobe S . Allatostatins: a growing family of neuropeptides with structural and functional diversity. Ann N Y Acad Sci. 2000; 897:311-29. DOI: 10.1111/j.1749-6632.1999.tb07902.x. View

Veenstra J, Costes L . Isolation and identification of a peptide and its cDNA from the mosquito Aedes aegypti related to Manduca sexta allatotropin. Peptides. 1999; 20(10):1145-51. DOI: 10.1016/s0196-9781(99)00117-5. View

Li Y, Unnithan G, Veenstra J, Feyereisen R, Noriega F . Stimulation of JH biosynthesis by the corpora allata of adult female Aedes aegypti in vitro: effect of farnesoic acid and Aedes allatotropin. J Exp Biol. 2003; 206(Pt 11):1825-32. DOI: 10.1242/jeb.00371. View

Gilbert L, Granger N, Roe R . The juvenile hormones: historical facts and speculations on future research directions. Insect Biochem Mol Biol. 2000; 30(8-9):617-44. DOI: 10.1016/s0965-1748(00)00034-5. View

10.

Stay B . A review of the role of neurosecretion in the control of juvenile hormone synthesis: a tribute to Berta Scharrer. Insect Biochem Mol Biol. 2000; 30(8-9):653-62. DOI: 10.1016/s0965-1748(00)00036-9. View

11.

Li Y, Hernandez-Martinez S, Unnithan G, Feyereisen R, Noriega F . Activity of the corpora allata of adult female Aedes aegypti: effects of mating and feeding. Insect Biochem Mol Biol. 2003; 33(12):1307-15. DOI: 10.1016/j.ibmb.2003.07.003. View

12.

Li Y, Hernandez-Martinez S, Noriega F . Inhibition of juvenile hormone biosynthesis in mosquitoes: effect of allatostatic head factors, PISCF- and YXFGL-amide-allatostatins. Regul Pept. 2004; 118(3):175-82. DOI: 10.1016/j.regpep.2003.12.004. View

13.

Feyereisen R, Tobe S . A rapid partition assay for routine analysis of juvenile hormone release by insect corpora allata. Anal Biochem. 1981; 111(2):372-5. DOI: 10.1016/0003-2697(81)90575-3. View

14.

Bennett H, Browne C, Solomon S . Purification of the two major forms of rat pituitary corticotropin using only reversed-phase liquid chromatography. Biochemistry. 1981; 20(16):4530-8. DOI: 10.1021/bi00519a004. View

15.

Feyereisen R, Farnsworth D . Precursor supply for insect juvenile hormone III biosynthesis in a cockroach. J Biol Chem. 1987; 262(6):2676-81. View

16.

PRATT G, Farnsworth D, Feyereisen R . Changes in the sensitivity of adult cockroach corpora allata to a brain allatostatin. Mol Cell Endocrinol. 1990; 70(2):185-95. DOI: 10.1016/0303-7207(90)90158-5. View

17.

Kramer S, Toschi A, Miller C, Kataoka H, Quistad G, Li J . Identification of an allatostatin from the tobacco hornworm Manduca sexta. Proc Natl Acad Sci U S A. 1991; 88(21):9458-62. PMC: 52737. DOI: 10.1073/pnas.88.21.9458. View

18.

Sutherland T, Feyereisen R . Target of cockroach allatostatin in the pathway of juvenile hormone biosynthesis. Mol Cell Endocrinol. 1996; 120(2):115-23. DOI: 10.1016/0303-7207(96)03825-7. View

19.

Noriega F, Wells M . A comparison of three methods for isolating RNA from mosquitoes. Insect Mol Biol. 1993; 2(1):21-4. DOI: 10.1111/j.1365-2583.1993.tb00121.x. View

20.

Veenstra J, Noriega F, Graf R, Feyereisen R . Identification of three allatostatins and their cDNA from the mosquito Aedes aegypti. Peptides. 1997; 18(7):937-42. DOI: 10.1016/s0196-9781(97)00032-6. View