Standards in Gene Expression Microarray Experiments

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2006 Aug 31

PMID 16939786

Citations 7

Authors

Marc Salit

Affiliations

Soon will be listed here.

Abstract

The use of standards in gene expression measurements with DNA microarrays is ubiquitous--they just are not yet the kind of standards that have yielded microarray gene expression profiles that can be readily compared across different studies and different laboratories. They also are not yet enabling microarray measurements of the known, verifiable quality needed so they can be used with confidence in genomic medicine in regulated environments.

Citing Articles

Microarray Scanner Performance Over a Five-Week Period as Measured With Cy5 and Cy3 Serial Dilution Slides.

Satterfield M, Lippa K, Lu Z, Salit M J Res Natl Inst Stand Technol. 2016; 113(3):157-74.

PMID: 27096118 PMC: 4654599. DOI: 10.6028/jres.113.012.

Selective nucleic acid capture with shielded covalent probes.

Vieregg J, Nelson H, Stoltz B, Pierce N J Am Chem Soc. 2013; 135(26):9691-9.

PMID: 23745667 PMC: 3703666. DOI: 10.1021/ja4009216.

Data standards for Omics data: the basis of data sharing and reuse.

Chervitz S, Deutsch E, Field D, Parkinson H, Quackenbush J, Rocca-Serra P Methods Mol Biol. 2011; 719:31-69.

PMID: 21370078 PMC: 4152841. DOI: 10.1007/978-1-61779-027-0_2.

Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells.

Shimada H, Obayashi T, Takahashi N, Matsui M, Sakamoto A Plant Methods. 2010; 6:29.

PMID: 21190547 PMC: 3023742. DOI: 10.1186/1746-4811-6-29.

Exploring the use of internal and externalcontrols for assessing microarray technical performance.

Lippa K, Duewer D, Salit M, Game L, Causton H BMC Res Notes. 2010; 3:349.

PMID: 21189145 PMC: 3020182. DOI: 10.1186/1756-0500-3-349.