Critical Role of Cortactin in Actin Ring Formation and Osteoclastic Bone Resorption

Overview

Journal J Bone Miner Metab

Specialty Endocrinology

Date 2006 Aug 29

PMID 16937268

Citations 14

Authors

Takuma Matsubara

Akira Myoui

Fumiyo Ikeda

Kenji Hata

Hideki Yoshikawa

Riko Nishimura

Toshiyuki Yoneda

Affiliations

Soon will be listed here.

Abstract

Tyrosine kinase c-Src plays an essential role in ruffled border formation and bone resorption in osteoclasts; however, it is unclear how c-Src controls ruffled border formation during bone resorption. To address this question, we investigated the role of cortactin, a c-Src substrate, in osteoclasts. We found that cortactin showed colocalization with c-Src and actin rings in osteoclasts. Overexpression of cortactin stimulated actin ring formation in RAW 264.7 cells. In contrast, overexpression of Csk inhibited tyrosine phosphorylation of cortactin and binding of cortactin to c-Src. More importantly, overexertion of a mutant cortactin strongly suppressed actin ring formation and bone resorbing activity in osteoclasts. Collectively, our data indicate that cortactin controls osteoclastic bone resorption by regulating actin organization.

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