Spatiotemporal Pattern of Neuroinflammation After Impact-acceleration Closed Head Injury in the Rat

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2006 Jul 26

PMID 16864909

Citations 9

Authors

Servan Rooker

Sebastian Jander

Jos Van Reempts

Guido Stoll

Philippe G Jorens

Marcel Borgers

Jan Verlooy

Affiliations

Soon will be listed here.

Abstract

Inflammatory processes have been implicated in the pathogenesis of traumatic brain damage. We analyzed the spatiotemporal expression pattern of the proinflammatory key molecules: interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, and inducible nitric oxide synthase in a rat closed head injury (CHI) paradigm. 51 rats were used for RT-PCR analysis after CHI, and 18 for immunocytochemistry. We found an early upregulation of IL-1beta, IL-6, and TNF-alpha mRNA between 1h and 7h after injury; the expression of iNOS mRNA only revealed a significant increase at 4h. After 24h, the expression decreased towards baseline levels, and remained low until 7d after injury. Immunocytochemically, IL-1beta induction was localized to ramified microglia in areas surrounding the primary impact place as well as deeper brain structures. Our study shows rapid induction of inflammatory gene expression that exceeds by far the primary impact site and might therefore contribute to tissue damage at remote sites.

Citing Articles

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Adenosine kinase facilitated astrogliosis-induced cortical neuronal death in traumatic brain injury.

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