PRINS As an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2006 Jul 25

PMID 16861761

Citations 2

Authors

Franck Pellestor

Brigitte Andreo

Tal Anahory

Samir Hamamah

Affiliations

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Abstract

An ultrarapid three- and four-color primed in situ (PRINS) procedure has been developed for rapid chromosome identification and aneuploidy assessment on isolated cells. Based on the direct in situ mixing of fluorochromes (fluorescein isothiocyanate, tetramethylrhodamine isothiocyanate, Cascade Blue), this multicolor PRINS procedure is described on unfertilized human oocytes and isolated human blastomeres.

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