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A Method for High-throughput Gene Expression Signature Analysis

Overview
Journal Genome Biol
Specialties Biology
Genetics
Date 2006 Jul 25
PMID 16859521
Citations 111
Authors
David Peck
Emily D Crawford
Kenneth N Ross
Kimberly Stegmaier
Todd R Golub
Justin Lamb
Affiliations
Soon will be listed here.
Abstract

Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.

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