» Articles » PMID: 16854461

Nano-fibrous Scaffolding Promotes Osteoblast Differentiation and Biomineralization

Overview
Journal Biomaterials
Date 2006 Jul 21
PMID 16854461
Citations 101
Authors
Affiliations
Soon will be listed here.
Abstract

Nano-fibrous poly(L-lactic acid) (PLLA) scaffolds with interconnected pores were developed under the hypothesis that nano-fibrous scaffolding would mimic a morphological function of collagen fibrils to create a more favorable microenvironment for cells versus solid-walled scaffolds. In this study, an in vitro system was used to examine biological properties of the nano-fibrous scaffolds compared with those of solid-walled scaffolds for their potential use in bone tissue engineering. Biomineralization was enhanced substantially on the nano-fibrous scaffolds compared to solid-walled scaffolds, and this was confirmed by von Kossa staining, measurement of calcium contents, and transmission electron microscopy. In support of this finding, osteoblasts cultured on the nano-fibrous scaffolds exhibited higher alkaline phosphatase activity and an earlier and enhanced expression of the osteoblast phenotype versus solid-walled scaffolds. Most notable were the increases in runx2 protein and in bone sialoprotein mRNA in cells cultured on nano-fibrous scaffolds versus solid-walled scaffolds. At the day 1 of culture, alpha2 and beta1 integrins as well as alphav and beta3 integrins were highly expressed on the surface of cells seeded on nano-fibrous scaffolds, and linked to this were higher levels of phospho-Paxillin and phospho-FAK in cell lysates. In contrast, cells seeded on solid-walled scaffolds expressed significantly lower levels of these integrins, phospho-Paxillin, and phospho-FAK. To further examine the role of nano-fibrous architecture, we inhibited the formation of collagen fibrils by adding 3,4-dehydroproline to cultures and then assayed cells for expression of alpha2 integrin. Cells seeded on nano-fibrous scaffolds sustained expression of alpha2 integrin in the presence of dehydroproline, while suppression of alpha2 integrin was evident in cells seeded on solid-walled scaffolds. These results provide initial evidence that synthetic nano fibers may exhibit certain properties that are comparable to natural collagen fibers, and thus, the nano-fibrous architecture may serve as a superior scaffolding versus solid-walled architecture for promoting osteoblast differentiation and biomineralization.

Citing Articles

Synthetic helical peptides on nanofibers to activate cell-surface receptors and synergistically enhance critical-sized bone defect regeneration.

Zhou T, C Cavalcante R, Ge C, Franceschi R, Ma P Bioact Mater. 2024; 43:98-113.

PMID: 39381328 PMC: 11458538. DOI: 10.1016/j.bioactmat.2024.08.017.


Enhancing bone tissue engineering using iron nanoparticles and magnetic fields: A focus on cytomechanics and angiogenesis in the chicken egg chorioallantoic membrane model.

Nelogi S, Patil A, Chowdhary R J Indian Prosthodont Soc. 2024; 24(2):175-185.

PMID: 38650343 PMC: 11129814. DOI: 10.4103/jips.jips_440_23.


Extracellular Matrices as Bioactive Materials for In Situ Tissue Regeneration.

Zhao P, Yang F, Jia X, Xiao Y, Hua C, Xing M Pharmaceutics. 2023; 15(12).

PMID: 38140112 PMC: 10747903. DOI: 10.3390/pharmaceutics15122771.


Long non-coding RNA AC018926.2 regulates palmitic acid exposure-compromised osteogenic potential of periodontal ligament stem cells via the ITGA2/FAK/AKT pathway.

Qu H, Sun L, Li X, Liu F, Sun H, He X Cell Prolif. 2023; 56(8):e13411.

PMID: 36720715 PMC: 10392068. DOI: 10.1111/cpr.13411.


The effect of external magnetic field on osteogenic and antimicrobial behaviour of surface-functionalized custom titanium chamber with iron nanoparticles. A preliminary research.

Nelogi S, Patil A, Chowdhary R Odontology. 2022; 111(3):600-607.

PMID: 36447109 DOI: 10.1007/s10266-022-00769-7.