Phenotypic Plasticity of T Cell Progenitors Upon Exposure to Notch Ligands

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2006 Jul 19

PMID 16847069

Citations 21

Authors

Andreas Krueger

Annette I Garbe

Harald von Boehmer

Affiliations

Soon will be listed here.

Abstract

Despite many efforts, the nature of thymic immigrants that give rise to T cells has remained obscure, especially since it became known that extrathymic lineage-negative, Sca-1-positive, c-kit high progenitor cells differ from intrathymic early T cell progenitors (ETPs) by functional potential and dependence on Notch signaling. After our observation that intrathymic T cell precursors expressing a human CD25 reporter under control of pre-TCRalpha regulatory elements almost exclusively have the ETP phenotype, we have analyzed the phenotypic changes of reporter-expressing common lymphoid progenitor (CLP) cells in the bone marrow when cultured on Delta-like 1-expressing stromal cells. We note that these quickly adopt the phenotype of double negative (DN)2 thymocytes with little display of the ETP phenotype. Our data suggest that common lymphoid progenitor (CLP) cells could be responsible for the rapid reconstitution of thymus function after bone marrow transplantation since CLP cells in the blood have the capacity to rapidly enter the thymus and become DN2 thymocytes.

Citing Articles

T Cell Development: From T-Lineage Specification to Intrathymic Maturation.

Golzari-Sorkheh M, Yoganathan K, Chen E, Singh J, Zuniga-Pflucker J Adv Exp Med Biol. 2025; 1471:81-137.

PMID: 40067585 DOI: 10.1007/978-3-031-77921-3_4.

The thymus road to a T cell: migration, selection, and atrophy.

Ruiz Perez M, Vandenabeele P, Tougaard P Front Immunol. 2024; 15:1443910.

PMID: 39257583 PMC: 11384998. DOI: 10.3389/fimmu.2024.1443910.

NOTCH1- and CD117-Positive Stem Cells in Human Endometriosis and Adenomyosis Lesions.

Metodiev D, Parvanov D, Ruseva M, Ganeva R, Handzhiyska M, Vidolova N Diagnostics (Basel). 2024; 14(15).

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Phosphate Transporter Profiles in Murine and Human Thymi Identify Thymocytes at Distinct Stages of Differentiation.

Machado A, Pouzolles M, Gailhac S, Fritz V, Craveiro M, Lopez-Sanchez U Front Immunol. 2020; 11:1562.

PMID: 32793218 PMC: 7387685. DOI: 10.3389/fimmu.2020.01562.

Multicongenic fate mapping quantification of dynamics of thymus colonization.

Zietara N, Lyszkiewicz M, Puchalka J, Witzlau K, Reinhardt A, Forster R J Exp Med. 2015; 212(10):1589-601.

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