The Nucleolus: a Model for the Organization of Nuclear Functions

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 2006 Jul 13

PMID 16835752

Citations 57

Authors

Daniele Hernandez-Verdun

Affiliations

Soon will be listed here.

Abstract

Nucleoli are the prominent contrasted structures of the cell nucleus. In the nucleolus, ribosomal RNAs (rRNAs) are synthesized, processed and assembled with ribosomal proteins. The size and organization of the nucleolus are directly related to ribosome production. The organization of the nucleolus reveals the functional compartmentation of the nucleolar machineries that depends on nucleolar activity. When this activity is blocked, disrupted or impossible, the nucleolar proteins have the capacity to interact independently of the processing activity. In addition, nucleoli are dynamic structures in which nucleolar proteins rapidly associate and dissociate with nucleolar components in continuous exchanges with the nucleoplasm. At the time of nucleolar assembly, the processing machineries are recruited in a regulated manner in time and space, controlled by different kinases and form intermediate structures, the prenucleolar bodies. The participation of stable pre-rRNAs in nucleolar assembly was demonstrated after mitosis and during development but this is an intriguing observation since the role of these pre-rRNAs is presently unknown. A brief report on the nucleolus and diseases is proposed as well as of nucleolar functions different from ribosome biogenesis.

Citing Articles

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References

Weisenberger D, Scheer U . A possible mechanism for the inhibition of ribosomal RNA gene transcription during mitosis. J Cell Biol. 1995; 129(3):561-75. PMC: 2120438. DOI: 10.1083/jcb.129.3.561. View

Rubbi C, Milner J . Disruption of the nucleolus mediates stabilization of p53 in response to DNA damage and other stresses. EMBO J. 2003; 22(22):6068-77. PMC: 275437. DOI: 10.1093/emboj/cdg579. View

Savino T, de Mey J, Sibarita J, Hernandez-Verdun D . Nucleolar assembly of the rRNA processing machinery in living cells. J Cell Biol. 2001; 153(5):1097-110. PMC: 2174343. DOI: 10.1083/jcb.153.5.1097. View

Pestov D, Strezoska Z, Lau L . Evidence of p53-dependent cross-talk between ribosome biogenesis and the cell cycle: effects of nucleolar protein Bop1 on G(1)/S transition. Mol Cell Biol. 2001; 21(13):4246-55. PMC: 87085. DOI: 10.1128/MCB.21.13.4246-4255.2001. View

Hozak P, Cook P, Schofer C, Mosgoller W, Wachtler F . Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells. J Cell Sci. 1994; 107 ( Pt 2):639-48. DOI: 10.1242/jcs.107.2.639. View

RITOSSA F, Spiegelman S . LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965; 53:737-45. PMC: 221060. DOI: 10.1073/pnas.53.4.737. View

Dundr M, Misteli T, Olson M . The dynamics of postmitotic reassembly of the nucleolus. J Cell Biol. 2000; 150(3):433-46. PMC: 2175201. DOI: 10.1083/jcb.150.3.433. View

Carmo-Fonseca M, Campos I . To be or not to be in the nucleolus. Nat Cell Biol. 2000; 2(6):E107-12. DOI: 10.1038/35014078. View

Snaar S, Wiesmeijer K, Jochemsen A, Tanke H, Dirks R . Mutational analysis of fibrillarin and its mobility in living human cells. J Cell Biol. 2000; 151(3):653-62. PMC: 2185578. DOI: 10.1083/jcb.151.3.653. View

10.

Dundr M, Hebert M, Karpova T, Stanek D, Xu H, Shpargel K . In vivo kinetics of Cajal body components. J Cell Biol. 2004; 164(6):831-42. PMC: 1630494. DOI: 10.1083/jcb.200311121. View

11.

Knibiehler B, Mirre C, Navarro A, Rosset R . Studies on chromatin organization in a nucleolus without fibrillar centres. Presence of a sub-nucleolar structure in KCo cells of Drosophila. Cell Tissue Res. 1984; 236(2):279-88. DOI: 10.1007/BF00214228. View

12.

Knibiehler B, Mirre C, Rosset R . Nucleolar organizer structure and activity in a nucleolus without fibrillar centres: the nucleolus in an established Drosophila cell line. J Cell Sci. 1982; 57:351-64. DOI: 10.1242/jcs.57.1.351. View

13.

Angelier N, Tramier M, Louvet E, Coppey-Moisan M, Savino T, De Mey J . Tracking the interactions of rRNA processing proteins during nucleolar assembly in living cells. Mol Biol Cell. 2005; 16(6):2862-71. PMC: 1142430. DOI: 10.1091/mbc.e05-01-0041. View

14.

Verheggen C, Almouzni G, Hernandez-Verdun D . The ribosomal RNA processing machinery is recruited to the nucleolar domain before RNA polymerase I during Xenopus laevis development. J Cell Biol. 2000; 149(2):293-306. PMC: 2175160. DOI: 10.1083/jcb.149.2.293. View

15.

Ruggero D, Pandolfi P . Does the ribosome translate cancer?. Nat Rev Cancer. 2003; 3(3):179-92. DOI: 10.1038/nrc1015. View

16.

Fomproix N, Hernandez-Verdun D . Effects of anti-fibrillarin antibodies on building of functional nucleoli at the end of mitosis. J Cell Sci. 1998; 111 ( Pt 3):359-72. DOI: 10.1242/jcs.111.3.359. View

17.

Scheer U, Benavente R . Functional and dynamic aspects of the mammalian nucleolus. Bioessays. 1990; 12(1):14-21. DOI: 10.1002/bies.950120104. View

18.

Grisendi S, Bernardi R, Rossi M, Cheng K, Khandker L, Manova K . Role of nucleophosmin in embryonic development and tumorigenesis. Nature. 2005; 437(7055):147-53. DOI: 10.1038/nature03915. View

19.

Thiry M, Lafontaine D . Birth of a nucleolus: the evolution of nucleolar compartments. Trends Cell Biol. 2005; 15(4):194-9. DOI: 10.1016/j.tcb.2005.02.007. View

20.

Louvet E, Junera H, Berthuy I, Hernandez-Verdun D . Compartmentation of the nucleolar processing proteins in the granular component is a CK2-driven process. Mol Biol Cell. 2006; 17(6):2537-46. PMC: 1474808. DOI: 10.1091/mbc.e05-10-0923. View