A Mediator-responsive Form of Metazoan RNA Polymerase II

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Jun 14

PMID 16769904

Citations 60

Authors

Xiaopeng Hu

Sohail Malik

Costin Catalin Negroiu

Kyle Hubbard

Chidambaram Natesa Velalar

Brian Hampton

Dan Grosu

Jennifer Catalano

Robert G Roeder

Averell Gnatt

Affiliations

Soon will be listed here.

Abstract

RNA polymerase II (Pol II), whose 12 subunits are conserved across eukaryotes, is at the heart of the machinery responsible for transcription of mRNA. Although associated general transcription factors impart promoter specificity, responsiveness to gene- and tissue-selective activators additionally depends on the multiprotein Mediator coactivator complex. We have isolated from tissue extracts a distinct and abundant mammalian Pol II subpopulation that contains an additional tightly associated polypeptide, Gdown1. Our results establish that Gdown1-containing Pol II, designated Pol II(G), is selectively dependent on and responsive to Mediator. Thus, in an in vitro assay with general transcription factors, Pol II lacking Gdown1 displays unfettered levels of activator-dependent transcription in the presence or absence of Mediator. In contrast, Pol II(G) is dramatically less efficient in responding to activators in the absence of Mediator yet is highly and efficiently responsive to activators in the presence of Mediator. Our results reveal a transcriptional control mechanism in which Mediator-dependent regulation is enforced by means of Gdown1, which likely restricts Pol II function only to be reversed by Mediator.

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