B Cell Antigen Receptor Signaling and Internalization Are Mutually Exclusive Events

Overview

Journal PLoS Biol

Specialty Biology

Date 2006 May 25

PMID 16719564

Citations 44

Authors

Ping Hou

Elizabeth Araujo

Tong Zhao

Miao Zhang

Don Massenburg

Margaret Veselits

Colleen Doyle

Aaron R Dinner

Marcus R Clark

Affiliations

Soon will be listed here.

Abstract

Engagement of the B cell antigen receptor initiates two concurrent processes, signaling and receptor internalization. While both are required for normal humoral immune responses, the relationship between these two processes is unknown. Herein, we demonstrate that following receptor ligation, a small subpopulation of B cell antigen receptors are inductively phosphorylated and selectively retained at the cell surface where they can serve as scaffolds for the assembly of signaling molecules. In contrast, the larger population of non-phosphorylated receptors is rapidly endocytosed. Each receptor can undergo only one of two mutually exclusive fates because the tyrosine-based motifs that mediate signaling when phosphorylated mediate internalization when not phosphorylated. Mathematical modeling indicates that the observed competition between receptor phosphorylation and internalization enhances signaling responses to low avidity ligands.

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