Conformation of the Synaptobrevin Transmembrane Domain

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 May 20

PMID 16709671

Citations 40

Authors

Mark Bowen

Axel T Brunger

Affiliations

Soon will be listed here.

Abstract

The synaptic vesicle protein synaptobrevin (also called VAMP, vesicle-associated membrane protein) forms part of the SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) complex, which is essential for vesicle fusion. Additionally, the synaptobrevin transmembrane domain can promote lipid mixing independently of complex formation. Here, the conformation of the transmembrane domain was studied by using circular dichroism and attenuated total reflection Fourier-transform infrared spectroscopy. The synaptobrevin transmembrane domain has an alpha-helical structure that breaks in the juxtamembrane region, leaving the cytoplasmic domain unstructured. In phospholipid bilayers, infrared dichroism data indicate that the transmembrane domain adopts a 36 degrees angle with respect to the membrane normal, similar to that reported for viral fusion peptides. A conserved aromatic/basic motif in the juxtamembrane region may be causing this relatively high insertion angle.

Citing Articles

Double-Transmembrane Domain of SNAREs Decelerates the Fusion by Increasing the Protein-Lipid Mismatch.

Bu B, Tian Z, Li D, Zhang K, Chen W, Ji B J Mol Biol. 2023; 435(13):168089.

PMID: 37030649 PMC: 10247502. DOI: 10.1016/j.jmb.2023.168089.

The function of VAMP2 in mediating membrane fusion: An overview.

Yan C, Jiang J, Yang Y, Geng X, Dong W Front Mol Neurosci. 2023; 15:948160.

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NMR identification of a conserved Drp1 cardiolipin-binding motif essential for stress-induced mitochondrial fission.

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Structural Roles for the Juxtamembrane Linker Region and Transmembrane Region of Synaptobrevin 2 in Membrane Fusion.

Hu Y, Zhu L, Ma C Front Cell Dev Biol. 2021; 8:609708.

PMID: 33490074 PMC: 7815645. DOI: 10.3389/fcell.2020.609708.

Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE.

Rathore S, Liu Y, Yu H, Wan C, Lee M, Yin Q Cell Rep. 2019; 29(13):4583-4592.e3.

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