The Role of Adipose Cell Size and Adipose Tissue Insulin Sensitivity in the Carbohydrate Intolerance of Human Obesity

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1968 Jan 1

PMID 16695937

Citations 174

Authors

L B Salans

J L Knittle

J Hirsch

Affiliations

Soon will be listed here.

Abstract

Glucose metabolism and insulin sensitivity of isolated human adipose tissue was studied as a function of adipose cell size and number. Glucose metabolism by these tissues was closely related to the number of cells in the fragment, irrespective of cell size. Adipose cells of obese individuals metabolized glucose to carbon dioxide and triglyceride at rates similar to adipose cells of nonobese subjects. In contrast, insulin responsiveness of adipose tissue was dependent upon adipose cell size. The larger its adipose cells the less insulin sensitive was the tissue. Thus, adipose tissue of obese subjects, with enlarged cells, showed a diminished response to insulin. After weight loss and reduction in adipose cell size, insulin sensitivity of the adipose tissue of obese patients was restored to normal. When adipose tissue of obese individuals showed impaired responsiveness to insulin, their plasma insulin levels, after oral glucose, were elevated. Weight loss and reduction in adipose cell size restored plasma insulin concentration to normal, concomitant with the return of normal tissue insulin sensitivity.

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References

Hellman B, Westman S . PALMITATE UTILIZATION IN OBESE-HYPERGLYCEMIC MICE. IN VITRO STUDIES OF EPIDIDYMAL ADIPOSE TISSUE AND LIVER. Acta Physiol Scand. 1964; 61:65-72. DOI: 10.1111/j.1748-1716.1964.tb02943.x. View

Beck P, KOUMANS J, WINTERLING C, Stein M, Daughaday W, KIPNIS D . STUDIES OF INSULIN AND GROWTH HORMONE SECRETION IN HUMAN OBESITY. J Lab Clin Med. 1964; 64:654-67. View

CONN J, FAJANS S . The prediabetic state. A concept of dynamic resistance to a genetic diabetogenic influence. Am J Med. 1961; 31:839-50. DOI: 10.1016/0002-9343(61)90023-7. View

Bjorntorp P, MARTINSSON A . The composition of human subcutaneous adipose tissue in relation to its morphology. Acta Med Scand. 1966; 179(4):475-81. DOI: 10.1111/j.0954-6820.1966.tb05485.x. View

Alp H, RECANT L . EFFECT OF THE INSULIN-INHIBITORY ALBUMIN FRACTION FROM NORMAL AND DIABETIC SUBJECTS ON ADIPOSE TISSUE. Metabolism. 1964; 13:609-19. DOI: 10.1016/0026-0495(64)90069-1. View

Di Girolamo M, RUDMAN D . Species differences in glucose metabolism and insulin responsiveness of adipose tissue. Am J Physiol. 1966; 210(4):721-7. DOI: 10.1152/ajplegacy.1966.210.4.721. View

BUTTERFIELD W, Hanley T, Whichelow M . PERIPHERAL METABOLISM OF GLUCOSE AND FREE FATTY ACIDS DURING ORAL GLUCOSE TOLERANCE TESTS. Metabolism. 1965; 14:851-66. DOI: 10.1016/0026-0495(65)90122-8. View

Lowy C, Blanshard G, PHEAR D . Antagonism of insulin by albumin. Lancet. 1961; 1(7181):802-4. DOI: 10.1016/s0140-6736(61)90121-0. View

Samaan N, Fraser R, DEMPSTER W . THE "TYPICAL" AND "ATYPICAL" FORMS OF SERUM INSULIN. Diabetes. 1963; 12:339-48. DOI: 10.2337/diab.12.4.339. View

10.

RUDMAN D, Garcia L, DIGIROLAMO M, Shank P . Cleavage of bovine insulin by rat adipose tissue. Endocrinology. 1966; 78(1):169-85. DOI: 10.1210/endo-78-1-169. View

11.

Hirsch J, Farquhar J, AHRENS Jr E, Peterson M, STOFFEL W . Studies of adipose tissue in man. A microtechnic for sampling and analysis. Am J Clin Nutr. 1960; 8:499-511. DOI: 10.1093/ajcn/8.4.499. View

12.

DOLE V . A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956; 35(2):150-4. PMC: 438791. DOI: 10.1172/JCI103259. View

13.

Antoniades H, BOUGAS J, PYLE H . Studies on the state of insulin in blood. Examination of splenic, portal and peripheral blood serum of diabetic and nondiabetic subjects for "free" insulin and insulin complexes. N Engl J Med. 1962; 267:218-22. DOI: 10.1056/NEJM196208022670502. View

14.

OWEN Jr J, LINDSAY R, Gaskin J, HOLLIFIELD G . Response of human adipose tissue to endogenous serum insulin-like activity in vitro. Metabolism. 1967; 16(1):47-56. DOI: 10.1016/0026-0495(67)90158-8. View

15.

KARAM J, GRODSKY G, FORSHAM P . Excessive insulin response to glucose in obese subjects as measured by immunochemical assay. Diabetes. 1963; 12:197-204. DOI: 10.2337/diab.12.3.197. View

16.

Lesser G, Kumar I, Steele J . CHANGES IN BODY COMPOSITION WITH AGE. Ann N Y Acad Sci. 1963; 110:578-88. DOI: 10.1111/j.1749-6632.1963.tb15781.x. View

17.

Hirsch J, GOLDRICK R . SERIAL STUDIES ON THE METABOLISM OF HUMAN ADIPOSE TISSUE. I. LIPOGENESIS AND FREE FATTY ACID UPTAKE AND RELEASE IN SMALL ASPIRATED SAMPLES OF SUBCUTANEOUS FAT. J Clin Invest. 1964; 43:1776-92. PMC: 441979. DOI: 10.1172/JCI105052. View

18.

BOSHELL B, Barrett J, WILENSKY A, PATTON T . INSULIN RESISTANCE. RESPONSE TO INSULIN FROM VARIOUS ANIMAL SOURCES, INCLUDING HUMAN. Diabetes. 1964; 13:144-52. DOI: 10.2337/diab.13.2.144. View

19.

Rodbell M . METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964; 239:375-80. View

20.

GRODSKY G, KARAM J, PAVLATOS F, FORSHAM P . Reduction by phenformin of excessive insulin levels after glucose loading in obese and diabetic subjects. Metabolism. 1963; 12:278-86. View