The Zinc Containing Pro-apoptotic Protein Siva Interacts with the Peroxisomal Membrane Protein Pmp22

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2006 May 10

PMID 16683188

Citations 3

Authors

Matthias Nestler

Ulrike Martin

Peter Hortschansky

Hans-Peter Saluz

Andreas Henke

Thomas Munder

Affiliations

Soon will be listed here.

Abstract

Host answers to pathogen attacks define the course of pathogenic events and decide about the fate of the host organism. Infection with coxsackievirus B3 (CVB3) can induce severe myocarditis and pancreatitis. The interplay between host factors and virus components is crucial for the fate of the infected host. As we have shown before, expression of the pro-apoptotic host protein Siva is significantly increased after CVB3 infection, and infected cells are removed by programmed cell death. Analysis of Siva expressed in Escherichia coli revealed that this protein binds three zinc ions, suggesting a rather complex three-dimensional structure. By screening a human heart cDNA library we found a new interaction partner of Siva. The peroxisomal membrane protein PMP22 may be involved in the host response against CVB3. Previous investigations showed that Siva interacts with the cytoplasmic C-terminus of CD27, a member of the tumor necrosis factor receptor group, and transmits an apoptotic signal. With the help of directed two-hybrid assays we determined the N-terminal part of Siva as the binding region for CD27.

Citing Articles

The N-terminal 33 amino acid domain of Siva-1 is sufficient for nuclear localization.

Chen J, Yang L, Huang Z Braz J Med Biol Res. 2013; 46(12):1021-1027.

PMID: 24345910 PMC: 3935273. DOI: 10.1590/1414-431X20132833.

The PMP22 gene and its related diseases.

Li J, Parker B, Martyn C, Natarajan C, Guo J Mol Neurobiol. 2012; 47(2):673-98.

PMID: 23224996 PMC: 3594637. DOI: 10.1007/s12035-012-8370-x.

Regulation of IL-2 gene expression by Siva and FOXP3 in human T cells.

Hench V, Su L BMC Immunol. 2011; 12:54.

PMID: 21955384 PMC: 3208582. DOI: 10.1186/1471-2172-12-54.

References

van den Bosch H, Schutgens R, Wanders R, Tager J . Biochemistry of peroxisomes. Annu Rev Biochem. 1992; 61:157-97. DOI: 10.1146/annurev.bi.61.070192.001105. View

Gravestein L, Blom B, Nolten L, de Vries E, Van Der Horst G, Ossendorp F . Cloning and expression of murine CD27: comparison with 4-1BB, another lymphocyte-specific member of the nerve growth factor receptor family. Eur J Immunol. 1993; 23(4):943-50. DOI: 10.1002/eji.1830230427. View

Clark M, Lieberman P, Berk A, Dasgupta A . Direct cleavage of human TATA-binding protein by poliovirus protease 3C in vivo and in vitro. Mol Cell Biol. 1993; 13(2):1232-7. PMC: 359008. DOI: 10.1128/mcb.13.2.1232-1237.1993. View

Gillum R . Idiopathic cardiomyopathy in the United States, 1970-1982. Am Heart J. 1986; 111(4):752-5. DOI: 10.1016/0002-8703(86)90111-0. View

Chu F, Borthakur A, Sun X, Barkinge J, Gudi R, Hawkins S . The Siva-1 putative amphipathic helical region (SAH) is sufficient to bind to BCL-XL and sensitize cells to UV radiation induced apoptosis. Apoptosis. 2004; 9(1):83-95. DOI: 10.1023/B:APPT.0000012125.01799.4c. View

Schwabe J, Klug A . Zinc mining for protein domains. Nat Struct Biol. 1994; 1(6):345-9. DOI: 10.1038/nsb0694-345. View

Phizicky E, Fields S . Protein-protein interactions: methods for detection and analysis. Microbiol Rev. 1995; 59(1):94-123. PMC: 239356. DOI: 10.1128/mr.59.1.94-123.1995. View

Huber S, Mortensen A, Moulton G . Modulation of cytokine expression by CD4+ T cells during coxsackievirus B3 infections of BALB/c mice initiated by cells expressing the gamma delta + T-cell receptor. J Virol. 1996; 70(5):3039-44. PMC: 190164. DOI: 10.1128/JVI.70.5.3039-3044.1996. View

Akiba H, Nakano H, Nishinaka S, Shindo M, Kobata T, Atsuta M . CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-kappaB and stress-activated protein kinase/c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-kappaB-inducing kinase. J Biol Chem. 1998; 273(21):13353-8. DOI: 10.1074/jbc.273.21.13353. View

10.

Hintzen R, Lens S, Lammers K, Kuiper H, Beckmann M, van Lier R . Engagement of CD27 with its ligand CD70 provides a second signal for T cell activation. J Immunol. 1995; 154(6):2612-23. View

11.

Sugrue D, Rodeheffer R, Codd M, Ballard D, Fuster V, Gersh B . The clinical course of idiopathic dilated cardiomyopathy. A population-based study. Ann Intern Med. 1992; 117(2):117-23. DOI: 10.7326/0003-4819-117-2-117. View

12.

Watts T . TNF/TNFR family members in costimulation of T cell responses. Annu Rev Immunol. 2005; 23:23-68. DOI: 10.1146/annurev.immunol.23.021704.115839. View

13.

Hampton M, Orrenius S . Dual regulation of caspase activity by hydrogen peroxide: implications for apoptosis. FEBS Lett. 1997; 414(3):552-6. DOI: 10.1016/s0014-5793(97)01068-5. View

14.

Henke A, Huber S, Stelzner A, Whitton J . The role of CD8+ T lymphocytes in coxsackievirus B3-induced myocarditis. J Virol. 1995; 69(11):6720-8. PMC: 189582. DOI: 10.1128/JVI.69.11.6720-6728.1995. View

15.

Olivetti G, Abbi R, Quaini F, Kajstura J, Cheng W, Nitahara J . Apoptosis in the failing human heart. N Engl J Med. 1997; 336(16):1131-41. DOI: 10.1056/NEJM199704173361603. View

16.

Gebhard J, Perry C, Harkins S, Lane T, Mena I, Asensio V . Coxsackievirus B3-induced myocarditis: perforin exacerbates disease, but plays no detectable role in virus clearance. Am J Pathol. 1998; 153(2):417-28. PMC: 1852975. DOI: 10.1016/S0002-9440(10)65585-X. View

17.

Simizu S, Takada M, Umezawa K, Imoto M . Requirement of caspase-3(-like) protease-mediated hydrogen peroxide production for apoptosis induced by various anticancer drugs. J Biol Chem. 1998; 273(41):26900-7. DOI: 10.1074/jbc.273.41.26900. View

18.

Mena I, Perry C, Harkins S, Rodriguez F, Gebhard J, Whitton J . The role of B lymphocytes in coxsackievirus B3 infection. Am J Pathol. 1999; 155(4):1205-15. PMC: 1867001. DOI: 10.1016/S0002-9440(10)65223-6. View

19.

Fields S . The future is function. Nat Genet. 1997; 15(4):325-7. DOI: 10.1038/ng0497-325. View

20.

Brosius U, Dehmel T, Gartner J . Two different targeting signals direct human peroxisomal membrane protein 22 to peroxisomes. J Biol Chem. 2001; 277(1):774-84. DOI: 10.1074/jbc.M108155200. View