Localization of the Escherichia Coli RNA Polymerase Beta' Subunit Residue Phosphorylated by Bacteriophage T7 Kinase Gp0.7

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2006 May 5

PMID 16672600

Citations 21

Authors

Elena Severinova

Konstantin Severinov

Affiliations

Soon will be listed here.

Abstract

During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of beta'. Using a phosphomimetic substitution of Thr(1068), we show that phosphorylation of beta' leads to increased rho-dependent transcription termination, which may help to switch from host to viral RNA polymerase transcription during phage development.

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