Genetic Diversity and Quinolone Resistance in Campylobacter Jejuni Isolates from Poultry in Senegal

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2006 May 5

PMID 16672471

Citations 23

Authors

Alfred Dieudonne Kinana

Eric Cardinale

Fatou Tall

Ibrahim Bahsoun

Jean-Marie Sire

Benoit Garin

Sebastien Breurec

Cheikh Saad-Bouh Boye

Jean-David Perrier-Gros-Claude

Affiliations

Soon will be listed here.

Abstract

We used the multilocus sequence typing (MLST) method to evaluate the genetic diversity of 46 Campylobacter jejuni isolates from chickens and to determine the link between quinolone resistance and sequence type (ST). There were a total of 16 ST genotypes, and the majority of them belonged to seven clonal complexes previously identified by using isolates from human disease. The ST-353 complex was the most common complex, whereas the ST-21, ST-42, ST-52, and ST-257 complexes were less well represented. The resistance phenotype varied for each ST, and the Thr-86-Ile substitution in the GyrA protein was the predominant mechanism of resistance to quinolone. Nine of the 14 isolates having the Thr-86-Ile substitution belonged to the ST-353 complex. MLST showed that the emergence of quinolone resistance is not related to the diffusion of a unique clone and that there is no link between ST genotype and quinolone resistance. Based on silent mutations, different variants of the gyrA gene were shown to exist for the same ST. These data provide useful information for understanding the epidemiology of C. jejuni in Senegal.

Citing Articles

Population Structure and Antimicrobial Resistance in Campylobacter jejuni and C. coli Isolated from Humans with Diarrhea and from Poultry, East Africa.

French N, Thomas K, Amani N, Benschop J, Bigogo G, Cleaveland S Emerg Infect Dis. 2024; 30(10):2079-2089.

PMID: 39320160 PMC: 11431929. DOI: 10.3201/eid3010.231399.

Transmission of dominant strains of Campylobacter jejuni and Campylobacter coli between farms and retail stores in Ecuador: Genetic diversity and antimicrobial resistance.

Montero L, Medina-Santana J, Ishida M, Sauders B, Trueba G, Vinueza-Burgos C PLoS One. 2024; 19(9):e0308030.

PMID: 39316598 PMC: 11421796. DOI: 10.1371/journal.pone.0308030.

Genomic diversity of Campylobacter jejuni and Campylobacter coli isolated from the Ethiopian dairy supply chain.

Admasie A, Wei X, Johnson B, Burns L, Pawar P, Aurand-Cravens A PLoS One. 2024; 19(8):e0305581.

PMID: 39159178 PMC: 11332940. DOI: 10.1371/journal.pone.0305581.

Whole Resistome Analysis in and Genomes Available in Public Repositories.

Cobo-Diaz J, Gonzalez Del Rio P, Alvarez-Ordonez A Front Microbiol. 2021; 12:662144.

PMID: 34290678 PMC: 8287256. DOI: 10.3389/fmicb.2021.662144.

Campylobacter jejuni isolated from poultry meat in Brazil: in silico analysis and genomic features of two strains with different phenotypes of antimicrobial susceptibility.

Rauber Wurfel S, Jorge S, de Oliveira N, Kremer F, Sanchez C, Campos V Mol Biol Rep. 2019; 47(1):671-681.

PMID: 31749118 DOI: 10.1007/s11033-019-05174-y.

References

Cardinale E, Dromigny J, Tall F, Ndiaye M, Konte M, Perrier-Gros-Claude J . Fluoroquinolone susceptibility of Campylobacter strains, Senegal. Emerg Infect Dis. 2004; 9(11):1479-81. DOI: 10.3201/eid0911.020693. View

Moore J, ORiordan L, Wareing D, Doyle R, Lanser J, Stanley T . Phenotypic and genotypic relationship between Campylobacter spp isolated from humans and chickens in Northern Ireland--a comparison of three phenotyping and two genotyping schemes. Int J Hyg Environ Health. 2003; 206(3):211-6. DOI: 10.1078/1438-4639-00215. View

Duim B, Godschalk P, van den Braak N, Dingle K, Dijkstra J, Leyde E . Molecular evidence for dissemination of unique Campylobacter jejuni clones in Curaçao, Netherlands Antilles. J Clin Microbiol. 2003; 41(12):5593-7. PMC: 309031. DOI: 10.1128/JCM.41.12.5593-5597.2003. View

Rozas J, Sanchez-DelBarrio J, Messeguer X, Rozas R . DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics. 2003; 19(18):2496-7. DOI: 10.1093/bioinformatics/btg359. View

Colles F, Jones K, Harding R, Maiden M . Genetic diversity of Campylobacter jejuni isolates from farm animals and the farm environment. Appl Environ Microbiol. 2003; 69(12):7409-13. PMC: 309911. DOI: 10.1128/AEM.69.12.7409-7413.2003. View

Stanley K, Jones K . Cattle and sheep farms as reservoirs of Campylobacter. J Appl Microbiol. 2003; 94 Suppl:104S-113S. DOI: 10.1046/j.1365-2672.94.s1.12.x. View

Rao M, Naficy A, Savarino S, Wierzba T, Peruski L, Frenck R . Pathogenicity and convalescent excretion of Campylobacter in rural Egyptian children. Am J Epidemiol. 2001; 154(2):166-73. DOI: 10.1093/aje/154.2.166. View

Bachoual R, Ouabdesselam S, Mory F, Lascols C, Soussy C, Tankovic J . Single or double mutational alterations of gyrA associated with fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli. Microb Drug Resist. 2002; 7(3):257-61. DOI: 10.1089/10766290152652800. View

Wassenaar T, Newell D . Genotyping of Campylobacter spp. Appl Environ Microbiol. 2000; 66(1):1-9. PMC: 91777. DOI: 10.1128/AEM.66.1.1-9.2000. View

10.

Schouls L, Reulen S, Duim B, Wagenaar J, Willems R, Dingle K . Comparative genotyping of Campylobacter jejuni by amplified fragment length polymorphism, multilocus sequence typing, and short repeat sequencing: strain diversity, host range, and recombination. J Clin Microbiol. 2003; 41(1):15-26. PMC: 149617. DOI: 10.1128/JCM.41.1.15-26.2003. View

11.

Blaser M . Epidemiologic and clinical features of Campylobacter jejuni infections. J Infect Dis. 1997; 176 Suppl 2:S103-5. DOI: 10.1086/513780. View

12.

Dingle K, Colles F, Ure R, Wagenaar J, Duim B, Bolton F . Molecular characterization of Campylobacter jejuni clones: a basis for epidemiologic investigation. Emerg Infect Dis. 2002; 8(9):949-55. PMC: 2732546. DOI: 10.3201/eid0809.020122. View

13.

Engberg J, Aarestrup F, Taylor D, Gerner-Smidt P, Nachamkin I . Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg Infect Dis. 2001; 7(1):24-34. PMC: 2631682. DOI: 10.3201/eid0701.010104. View

14.

Wang Y, Huang W, Taylor D . Cloning and nucleotide sequence of the Campylobacter jejuni gyrA gene and characterization of quinolone resistance mutations. Antimicrob Agents Chemother. 1993; 37(3):457-63. PMC: 187693. DOI: 10.1128/AAC.37.3.457. View

15.

Feil E, Li B, Aanensen D, Hanage W, Spratt B . eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol. 2004; 186(5):1518-30. PMC: 344416. DOI: 10.1128/JB.186.5.1518-1530.2004. View

16.

Jolley K, Feil E, Chan M, Maiden M . Sequence type analysis and recombinational tests (START). Bioinformatics. 2001; 17(12):1230-1. DOI: 10.1093/bioinformatics/17.12.1230. View

17.

Gillespie I, OBrien S, Frost J, Adak G, Horby P, Swan A . A case-case comparison of Campylobacter coli and Campylobacter jejuni infection: a tool for generating hypotheses. Emerg Infect Dis. 2002; 8(9):937-42. PMC: 2732536. DOI: 10.3201/eid0809.010817. View

18.

Suerbaum S, Lohrengel M, Sonnevend A, Ruberg F, Kist M . Allelic diversity and recombination in Campylobacter jejuni. J Bacteriol. 2001; 183(8):2553-9. PMC: 95172. DOI: 10.1128/JB.183.8.2553-2559.2001. View

19.

Butzler J . Campylobacter, from obscurity to celebrity. Clin Microbiol Infect. 2004; 10(10):868-76. DOI: 10.1111/j.1469-0691.2004.00983.x. View

20.

Manning G, Dowson C, Bagnall M, Ahmed I, West M, Newell D . Multilocus sequence typing for comparison of veterinary and human isolates of Campylobacter jejuni. Appl Environ Microbiol. 2003; 69(11):6370-9. PMC: 262249. DOI: 10.1128/AEM.69.11.6370-6379.2003. View