DNA Sequence Analysis of a Complementary DNA for Cold-Regulated Arabidopsis Gene Cor15 and Characterization of the COR 15 Polypeptide

Overview

Journal Plant Physiol

Specialty Physiology

Date 1992 Jun 1

PMID 16668917

Citations 64

Authors

C Lin

M F Thomashow

Affiliations

Soon will be listed here.

Abstract

Previous studies have indicated that changes in gene expression occur in Arabidopsis thaliana L. (Heyn) during cold acclimation and that certain of the cor (cold-regulated) genes encode polypeptides that share the unusual property of remaining soluble upon boiling in aqueous solution. Here, we identify a cDNA clone for a cold-regulated gene encoding one of the "boiling-stable" polypeptides, COR15. DNA sequence analysis indicated that the gene, designated cor15, encodes a 14.7-kilodalton hydrophilic polypeptide having an N-terminal amino acid sequence that closely resembles transit peptides that target proteins to the stromal compartment of chloroplasts. Immunological studies indicated that COR15 is processed in vivo and that the mature polypeptide, COR 15m, is present in the soluble fraction of chloroplasts. Possible functions of COR 15m are discussed.

Citing Articles

Cold stress induces differential gene expression of retained homeologs in cv Suneson.

Fang C, Hamilton J, Vaillancourt B, Wang Y, Wood J, Deans N Front Plant Sci. 2023; 14:1271625.

PMID: 38034564 PMC: 10687638. DOI: 10.3389/fpls.2023.1271625.

Chemical screening approach using single leaves identifies compounds that affect cold signaling in Arabidopsis.

Kitawaki K, Mihara R, Kamimura S, Sato A, Ushiyama M, Ito-Inaba Y Plant Physiol. 2023; 193(1):234-245.

PMID: 37177986 PMC: 10469520. DOI: 10.1093/plphys/kiad280.

Mechanism of in regulating cold tolerance of cucumber.

Yan Y, Mintao S, Si M, Qian F, Yijia W, Qinghua D Hortic Res. 2022; 9:uhac109.

PMID: 35821703 PMC: 9265480. DOI: 10.1093/hr/uhac109.

Low Fluence Ultraviolet-B Promotes Ultraviolet Resistance 8-Modulated Flowering in .

Zioutopoulou A, Patitaki E, ODonnell L, Kaiserli E Front Plant Sci. 2022; 13:840720.

PMID: 35432431 PMC: 9009151. DOI: 10.3389/fpls.2022.840720.

Fine-Tuning Cold Stress Response Through Regulated Cellular Abundance and Mechanistic Actions of Transcription Factors.

Abdullah S, Azzeme A, Yousefi K Front Plant Sci. 2022; 13:850216.

PMID: 35422820 PMC: 9002269. DOI: 10.3389/fpls.2022.850216.

References

Volger H, Heber U . Cryoprotective leaf proteins. Biochim Biophys Acta. 1975; 412(2):335-49. DOI: 10.1016/0005-2795(75)90048-3. View

Cox W . Interrelations between Environmental Factors and Freezing Resistance of Cabbage Leaves. Plant Physiol. 1976; 57(4):553-5. PMC: 542070. DOI: 10.1104/pp.57.4.553. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Gilmour S, Artus N, Thomashow M . cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana. Plant Mol Biol. 1992; 18(1):13-21. DOI: 10.1007/BF00018452. View

Close T, Kortt A, Chandler P . A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol. 1989; 13(1):95-108. DOI: 10.1007/BF00027338. View

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

Garnier J, Osguthorpe D, Robson B . Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol. 1978; 120(1):97-120. DOI: 10.1016/0022-2836(78)90297-8. View

Fawcett T, Browse J, Volokita M, Bartlett S . Spinach carbonic anhydrase primary structure deduced from the sequence of a cDNA clone. J Biol Chem. 1990; 265(10):5414-7. View

Steponkus P, Lynch D . Freeze/thaw-induced destabilization of the plasma membrane and the effects of cold acclimation. J Bioenerg Biomembr. 1989; 21(1):21-41. DOI: 10.1007/BF00762210. View

10.

Volokita M, Somerville C . The primary structure of spinach glycolate oxidase deduced from the DNA sequence of a cDNA clone. J Biol Chem. 1987; 262(33):15825-8. View

11.

SIMINOVITCH D, Cloutier Y . Drought and freezing tolerance and adaptation in plants: some evidence of near equivalences. Cryobiology. 1983; 20(4):487-503. DOI: 10.1016/0011-2240(83)90037-8. View

12.

Weiser C . Cold Resistance and Injury in Woody Plants: Knowledge of hardy plant adaptations to freezing stress may help us to reduce winter damage. Science. 1970; 169(3952):1269-78. DOI: 10.1126/science.169.3952.1269. View

13.

Hajela R, Horvath D, Gilmour S, Thomashow M . Molecular Cloning and Expression of cor (Cold-Regulated) Genes in Arabidopsis thaliana. Plant Physiol. 1990; 93(3):1246-52. PMC: 1062659. DOI: 10.1104/pp.93.3.1246. View

14.

Jacobsen J, Shaw D . Heat-stable proteins and abscisic Acid action in barley aleurone cells. Plant Physiol. 1989; 91(4):1520-6. PMC: 1062216. DOI: 10.1104/pp.91.4.1520. View

15.

Lin C, Guo W, Everson E, Thomashow M . Cold acclimation in Arabidopsis and wheat : a response associated with expression of related genes encoding ;boiling-stable' polypeptides. Plant Physiol. 1990; 94(3):1078-83. PMC: 1077344. DOI: 10.1104/pp.94.3.1078. View

16.

Mohapatra S, Wolfraim L, Poole R, Dhindsa R . Molecular cloning and relationship to freezing tolerance of cold-acclimation-specific genes of alfalfa. Plant Physiol. 1989; 89(1):375-80. PMC: 1055846. DOI: 10.1104/pp.89.1.375. View

17.

Gavel Y, von Heijne G . A conserved cleavage-site motif in chloroplast transit peptides. FEBS Lett. 1990; 261(2):455-8. DOI: 10.1016/0014-5793(90)80614-o. View

18.

Blake M, Johnston K, Russell-Jones G, Gotschlich E . A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem. 1984; 136(1):175-9. DOI: 10.1016/0003-2697(84)90320-8. View

19.

Schiffer M, EDMUNDSON A . Use of helical wheels to represent the structures of proteins and to identify segments with helical potential. Biophys J. 1967; 7(2):121-35. PMC: 1368002. DOI: 10.1016/S0006-3495(67)86579-2. View

20.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View