The Mechanism of Herbicide Resistance in Tobacco Cells with a New Mutation in the Q(b) Protein

Overview

Journal Plant Physiol

Specialty Physiology

Date 1989 Mar 1

PMID 16666653

Citations 4

Authors

Y Sigematsu

F Sato

Y Yamada

Affiliations

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Abstract

A new mutant of the psbA gene conferring resistance to 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine (atrazine) was obtained by selection of photomixotrophic tobacco (Nicotiana tabacum cv Samsun NN) cells. The 264th codon AGT (serine) in the wild psbA gene was changed to ACT (threonine) in these mutant tobacco cells. All other higher plants resistant to atrazine exhibit a change to GGT (glycine) in this codon. Measurements of Hill reaction activity and chlorophyll fluorescence showed that the threonine 264-containing plastoquinone serving as secondary stable electron acceptor of PSII (Q(B) protein) had not only strong resistance to triazine-type herbicides but also moderate resistance to substituted urea-type herbicides. Threonine-type Q(B) protein showed especially strong resistance to methoxylamino derivatives of the substituted urea herbicides. The projected secondary structures of the mutant Q(B) proteins indicate that the cross-resistance of threonine 264 Q(B) protein to triazine and urea herbicides is mainly due to a conformational change of the binding site for the herbicides. However, the glycine 264 Q(B) protein is resistant to only triazine herbicides because of the absence of an hydroxyl group and not because of a conformational change.

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References

McNally S, Bettini P, Sevignac M, Darmency H, Gasquez J, Dron M . A rapid method to test for chloroplast DNA involvement in atrazine resistance. Plant Physiol. 1987; 83(2):248-50. PMC: 1056341. DOI: 10.1104/pp.83.2.248. View

Hirschberg J, McIntosh L . Molecular Basis of Herbicide Resistance in Amaranthus hybridus. Science. 1983; 222(4630):1346-9. DOI: 10.1126/science.222.4630.1346. View

Golden S, Haselkorn R . Mutation to herbicide resistance maps within the psbA gene of Anacystis nidulans R2. Science. 1985; 229(4718):1104-7. DOI: 10.1126/science.3929379. View

Erickson J, Rahire M, Bennoun P, Delepelaire P, Diner B, Rochaix J . Herbicide resistance in Chlamydomonas reinhardtii results from a mutation in the chloroplast gene for the 32-kilodalton protein of photosystem II. Proc Natl Acad Sci U S A. 1984; 81(12):3617-21. PMC: 345269. DOI: 10.1073/pnas.81.12.3617. View

Nanba O, Satoh K . Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559. Proc Natl Acad Sci U S A. 1987; 84(1):109-12. PMC: 304151. DOI: 10.1073/pnas.84.1.109. View

Bettini P, McNally S, Sevignac M, Darmency H, Gasquez J, Dron M . Atrazine Resistance in Chenopodium album: Low and High Levels of Resistance to the Herbicide Are Related to the Same Chloroplast PSBA Gene Mutation. Plant Physiol. 1987; 84(4):1442-6. PMC: 1056793. DOI: 10.1104/pp.84.4.1442. View

Erickson J, Rahire M, Rochaix J, Mets L . Herbicide resistance and cross-resistance: changes at three distinct sites in the herbicide-binding protein. Science. 1985; 228(4696):204-7. DOI: 10.1126/science.228.4696.204. View

Pfister K, Steinback K, Gardner G, Arntzen C . Photoaffinity labeling of an herbicide receptor protein in chloroplast membranes. Proc Natl Acad Sci U S A. 1981; 78(2):981-5. PMC: 319929. DOI: 10.1073/pnas.78.2.981. View

Sato F, Shigematsu Y, Yamada Y . Selection of an atrazine-resistant tobacco cell line having a mutant psbA gene. Mol Gen Genet. 1988; 214(2):358-60. DOI: 10.1007/BF00337736. View

10.

Goloubinoff P, Edelman M, Hallick R . Chloroplast-coded atrazine resistance in Solanum nigrum: psbA loci from susceptible and resistant biotypes are isogenic except for a single codon change. Nucleic Acids Res. 1984; 12(24):9489-96. PMC: 320475. DOI: 10.1093/nar/12.24.9489. View

11.

Chou P, Fasman G . Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978; 47:45-148. DOI: 10.1002/9780470122921.ch2. View

12.

Michel H, Epp O, Deisenhofer J . Pigment-protein interactions in the photosynthetic reaction centre from Rhodopseudomonas viridis. EMBO J. 1986; 5(10):2445-51. PMC: 1167138. DOI: 10.1002/j.1460-2075.1986.tb04520.x. View

13.

Zurawski G, Bohnert H, Whitfeld P, Bottomley W . Nucleotide sequence of the gene for the M(r) 32,000 thylakoid membrane protein from Spinacia oleracea and Nicotiana debneyi predicts a totally conserved primary translation product of M(r) 38,950. Proc Natl Acad Sci U S A. 1982; 79(24):7699-703. PMC: 347415. DOI: 10.1073/pnas.79.24.7699. View

14.

Sayre R, Andersson B, Bogorad L . The topology of a membrane protein: the orientation of the 32 kd Qb-binding chloroplast thylakoid membrane protein. Cell. 1986; 47(4):601-8. DOI: 10.1016/0092-8674(86)90624-0. View

15.

Gardner G . Azidoatrazine: photoaffinity label for the site of triazine herbicide action in chloroplasts. Science. 1981; 211(4485):937-40. DOI: 10.1126/science.211.4485.937. View

16.

Arnon D . COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949; 24(1):1-15. PMC: 437905. DOI: 10.1104/pp.24.1.1. View