Flavan-3-ol Biosynthesis : The Conversion of (+)-Dihydroquercetin and Flavan-3,4-cis-Diol (Leucocyanidin) to (+)-Catechin by Reductases Extracted from Cell Suspension Cultures of Douglas Fir

Overview

Journal Plant Physiol

Specialty Physiology

Date 1984 Sep 1

PMID 16663794

Citations 17

Authors

H A Stafford

H H Lester

Affiliations

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Abstract

Extracts of cell suspension cultures from Douglas fir (Pseudotsuga menziesii) needles catalyzed the production of (+)-catechin (2,3-trans flavan-3-o1) from the 2,3-trans-flavan,3,4-cis-diol (leucocyanidin) in a NADPH-dependent reductase reaction at pH 7.4. Catechin was also produced, along with the 3,4-cis-diol, in a double step reduction from (+)-dihydroquercetin. It was necessary to eliminate any thiol such as 2-mercaptoethanol or dithiothreitol from the extract or assay mixture because these thiols apparently formed thioethers with the 3,4-diols.

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References

Stafford H, Lester H . Enzymic and nonenzymic reduction of (+)-dihydroquercetin to its 3,4,-diol. Plant Physiol. 1982; 70(3):695-8. PMC: 1065754. DOI: 10.1104/pp.70.3.695. View

Hagmann M, Heller W, Grisebach H . Induction and characterization of a microsomal flavonoid 3'-hydroxylase from parsley cell cultures. Eur J Biochem. 1983; 134(3):547-54. DOI: 10.1111/j.1432-1033.1983.tb07601.x. View