Synthesis of Nitrate Reductase in Chlorella: II. EVIDENCE FOR SYNTHESIS IN AMMONIA-GROWN CELLS

Overview

Journal Plant Physiol

Specialty Physiology

Date 1980 May 1

PMID 16661311

Citations 17

Authors

E A Funkhouser

Affiliations

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Abstract

Antiserum was prepared against nitrate reductase (EC 1.6.6.1) purified to homogeneity from Chlorella vulgaris Beijerinck. Both crude antiserum and anti-nitrate reductase antibodies prepared from it were used as re-agents to study the synthesis of nitrate reductase. Cell extracts from cultures which were grown with ammonia salts as the sole source of nitrogen contained almost no active enzyme. These extracts did contain material which binds to antibody and is thus immunologically related to purified nitrate reductase. The presence of this cross reacting material in cell extracts was detected by the ability of these extracts to (a) lower the titer of antisera; (b) form a biphasic precipitin curve with purified antibody; and (c) increase the peak height of a standard amount of purified nitrate reductase in rocket immunoelectrophoresis assay. These results suggest that ammonia-grown cells contain nitrate reductase precursor protein.

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References

Jackson A, Larkins B . Influence of Ionic Strength, pH, and Chelation of Divalent Metals on Isolation of Polyribosomes from Tobacco Leaves. Plant Physiol. 1976; 57(1):5-10. PMC: 541953. DOI: 10.1104/pp.57.1.5. View

Amy N, Garrett R . Immunoelectrophoretic determination of nitrate reductase in Neurospora crassa. Anal Biochem. 1979; 95(1):97-107. DOI: 10.1016/0003-2697(79)90191-x. View

Giri L, Ramadoss C . Physical studies on assimilatory nitrate reductase from Chlorella vulgaris. J Biol Chem. 1979; 254(22):11703-12. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Solomonson L, Vennesland B . Properties of a nitrate reductase of Chlorella. Biochim Biophys Acta. 1972; 267(3):544-57. DOI: 10.1016/0005-2728(72)90183-1. View

Solomonson L, Lorimer G, Hall R, Borchers R, Bailey J . Reduced nicotinamide adenine dinucleotide-nitrate reductase of Chlorella vulgaris. Purification, prosthetic groups, and molecular properties. J Biol Chem. 1975; 250(11):4120-7. View

Premakumar R, SORGER G, Gooden D . Stability of messenger RNA for nitrate reductase in Neurospora crassa. Biochim Biophys Acta. 1978; 519(1):275-8. DOI: 10.1016/0005-2787(78)90080-1. View

Jetschmann K, Solomonson L, Vennesland B . Activation of nitrate reductase by oxidation. Biochim Biophys Acta. 1972; 275(2):276-8. DOI: 10.1016/0005-2728(72)90048-5. View

Nicholas D, Ferrante J, Clarke G . Immunological studies with nitrogenase from Azotobacter and bacterial nitrate reductases. Anal Biochem. 1979; 95(1):24-31. DOI: 10.1016/0003-2697(79)90180-5. View

10.

Van t Riet J, Wientjes F, van Doorn J, Planta R . Purification and characterization of the respiratory nitrate reductase of Bacillus licheniformis. Biochim Biophys Acta. 1979; 576(2):347-60. DOI: 10.1016/0005-2795(79)90410-0. View

11.

Solomonson L . Purification of NADH-Nitrate Reductase by Affinity Chromatography. Plant Physiol. 1975; 56(6):853-5. PMC: 541940. DOI: 10.1104/pp.56.6.853. View

12.

Solomonson L, Vennesland B . Nitrate Reductase and Chlorate Toxicity in Chlorella vulgaris Beijerinck. Plant Physiol. 1972; 50(4):421-4. PMC: 366157. DOI: 10.1104/pp.50.4.421. View

13.

Notton B, Graf L, Hewitt E, Povey R . The role of molybdenum in the synthesis of nitrate reductase in cauliflower (Brassica oleracea L. var Botrytis L.) and spinach (Spinacea oleracea L.). Biochim Biophys Acta. 1974; 364(1):45-58. DOI: 10.1016/0005-2744(74)90131-4. View

14.

Losada M, Paneque A, Aparicio P, Vega J, Cardenas J, Herrera J . Inactivation and repression by ammonium of the nitrate reducing system in chlorella. Biochem Biophys Res Commun. 1970; 38(6):1009-15. DOI: 10.1016/0006-291x(70)90340-2. View

15.

Funkhouser E . Synthesis of Nitrate Reductase in Chlorella: I. EVIDENCE FOR AN INACTIVE PROTEIN PRECURSOR. Plant Physiol. 1980; 65(5):939-43. PMC: 440452. DOI: 10.1104/pp.65.5.939. View

16.

Vennesland B, Jetschmann C . The nitrate reductase of Chlorella pyrenoidosa. Biochim Biophys Acta. 1971; 227(3):554-64. DOI: 10.1016/0005-2744(71)90006-4. View