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Purification of NADH-Nitrate Reductase by Affinity Chromatography

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Journal Plant Physiol
Specialty Physiology
Date 1975 Dec 1
PMID 16659410
Citations 23
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Abstract

ASSIMILATORY NITRATE REDUCTASE (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of mumolar concentrations of NADH.

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