Formation of Adenosine 5'-phosphorofluoridate and the Assay of Adenyl Cyclase in Barley Seeds

Overview

Journal Plant Physiol

Specialty Physiology

Date 1974 Feb 1

PMID 16658665

Citations 3

Authors

R Alvarez

Affiliations

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Abstract

Barley seed (Hordeum vulgare L.) homogenates contain an apparent enzymatic activity which catalyzes the synthesis of adenosine 5'-phosphorofluoridate from magnesium-adenosine 5'-triphosphate and sodium fluoride. Formation of this compound may interfere with some adenyl cyclase assays which use fluoride as a component of the incubation medium. Neither adenyl cyclase activity nor endogenous adenosine 3': 5'-monophosphate was detected in barley seed homogenates or extracts.

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