Interaction of Moloney Murine Leukemia Virus Matrix Protein with IQGAP

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2006 Apr 22

PMID 16628219

Citations 36

Authors

Juliana Leung

Andrew Yueh

Frank S K Appah Jr

Bing Yuan

Kenia de Los Santos

Stephen P Goff

Affiliations

Soon will be listed here.

Abstract

The matrix protein (MA) of the Moloney murine leukemia virus (M-MuLV) was found to interact with IQGAP1, a prominent regulator of the cytoskeleton. Mutational studies defined residues of MA critical for the interaction, and tests of viruses carrying MA mutations revealed a near-perfect correlation between binding and virus replication. The replication-defective mutants showed defects in both early and late stages of the life cycle. Four viable second-site revertant viruses were isolated from three different replication-defective parental mutants, and in all cases the interaction with IQGAP1 was restored by the suppressor mutations. The interaction of MA and IQGAP1 was readily detected in vitro and in vivo. Virus replication was potently inhibited by a C-terminal fragment of IQGAP1, and impaired by RNAi knockdown of IQGAP1 and 2. We suggest that the IQGAPs link the virus to the cytoskeleton for trafficking both into and out of the cell.

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References

Li S, Wang Q, Chakladar A, Bronson R, Bernards A . Gastric hyperplasia in mice lacking the putative Cdc42 effector IQGAP1. Mol Cell Biol. 1999; 20(2):697-701. PMC: 85173. DOI: 10.1128/MCB.20.2.697-701.2000. View

Ho S, Hunt H, Horton R, Pullen J, Pease L . Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989; 77(1):51-9. DOI: 10.1016/0378-1119(89)90358-2. View

Fukata M, Kuroda S, Fujii K, Nakamura T, Shoji I, Matsuura Y . Regulation of cross-linking of actin filament by IQGAP1, a target for Cdc42. J Biol Chem. 1997; 272(47):29579-83. DOI: 10.1074/jbc.272.47.29579. View

Resh M . A myristoyl switch regulates membrane binding of HIV-1 Gag. Proc Natl Acad Sci U S A. 2004; 101(2):417-8. PMC: 327161. DOI: 10.1073/pnas.0308043101. View

Tang Y, Winkler U, Freed E, Torrey T, Kim W, Li H . Cellular motor protein KIF-4 associates with retroviral Gag. J Virol. 1999; 73(12):10508-13. PMC: 113106. DOI: 10.1128/JVI.73.12.10508-10513.1999. View

Weissbach L, Bernards A, Herion D . Binding of myosin essential light chain to the cytoskeleton-associated protein IQGAP1. Biochem Biophys Res Commun. 1998; 251(1):269-76. DOI: 10.1006/bbrc.1998.9371. View

Fukata M, Watanabe T, Noritake J, Nakagawa M, Yamaga M, Kuroda S . Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170. Cell. 2002; 109(7):873-85. DOI: 10.1016/s0092-8674(02)00800-0. View

Colicelli J, Goff S . Sequence and spacing requirements of a retrovirus integration site. J Mol Biol. 1988; 199(1):47-59. DOI: 10.1016/0022-2836(88)90378-6. View

Kuroda S, Fukata M, Kobayashi K, Nakafuku M, Nomura N, Iwamatsu A . Identification of IQGAP as a putative target for the small GTPases, Cdc42 and Rac1. J Biol Chem. 1996; 271(38):23363-7. DOI: 10.1074/jbc.271.38.23363. View

10.

Zhou W, Parent L, Wills J, Resh M . Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids. J Virol. 1994; 68(4):2556-69. PMC: 236733. DOI: 10.1128/JVI.68.4.2556-2569.1994. View

11.

Watanabe T, Wang S, Noritake J, Sato K, Fukata M, Takefuji M . Interaction with IQGAP1 links APC to Rac1, Cdc42, and actin filaments during cell polarization and migration. Dev Cell. 2004; 7(6):871-83. DOI: 10.1016/j.devcel.2004.10.017. View

12.

Fukata M, Kuroda S, Nakagawa M, Kawajiri A, Itoh N, Shoji I . Cdc42 and Rac1 regulate the interaction of IQGAP1 with beta-catenin. J Biol Chem. 1999; 274(37):26044-50. DOI: 10.1074/jbc.274.37.26044. View

13.

Kiernan R, Ono A, Englund G, Freed E . Role of matrix in an early postentry step in the human immunodeficiency virus type 1 life cycle. J Virol. 1998; 72(5):4116-26. PMC: 109641. DOI: 10.1128/JVI.72.5.4116-4126.1998. View

14.

Garrus J, von Schwedler U, Pornillos O, Morham S, Zavitz K, Wang H . Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell. 2001; 107(1):55-65. DOI: 10.1016/s0092-8674(01)00506-2. View

15.

Bacharach E, Gonsky J, ALIN K, Orlova M, Goff S . The carboxy-terminal fragment of nucleolin interacts with the nucleocapsid domain of retroviral gag proteins and inhibits virion assembly. J Virol. 2000; 74(23):11027-39. PMC: 113183. DOI: 10.1128/jvi.74.23.11027-11039.2000. View

16.

Besnier C, Takeuchi Y, Towers G . Restriction of lentivirus in monkeys. Proc Natl Acad Sci U S A. 2002; 99(18):11920-5. PMC: 129369. DOI: 10.1073/pnas.172384599. View

17.

Weissbach L, Settleman J, Kalady M, Snijders A, Murthy A, Yan Y . Identification of a human rasGAP-related protein containing calmodulin-binding motifs. J Biol Chem. 1994; 269(32):20517-21. View

18.

Yuan X, Yu X, Lee T, Essex M . Mutations in the N-terminal region of human immunodeficiency virus type 1 matrix protein block intracellular transport of the Gag precursor. J Virol. 1993; 67(11):6387-94. PMC: 238073. DOI: 10.1128/JVI.67.11.6387-6394.1993. View

19.

Basyuk E, Galli T, Mougel M, Blanchard J, Sitbon M, Bertrand E . Retroviral genomic RNAs are transported to the plasma membrane by endosomal vesicles. Dev Cell. 2003; 5(1):161-74. DOI: 10.1016/s1534-5807(03)00188-6. View

20.

Crawford S, Goff S . Mutations in gag proteins P12 and P15 of Moloney murine leukemia virus block early stages of infection. J Virol. 1984; 49(3):909-17. PMC: 255553. DOI: 10.1128/JVI.49.3.909-917.1984. View