» Articles » PMID: 16593377

Chlamydomonas Agglutinin is a Hydroxyproline-rich Glycoprotein

Overview
Specialty Science
Date 1983 Oct 1
PMID 16593377
Citations 28
Authors
Affiliations
Soon will be listed here.
Abstract

The mt(+) sexual agglutinin from Chlamydomonas reinhardi is shown to contain approximately 12% hydroxyproline, and two inhibitors of hydroxyproline formation, alpha,alpha'-dipyridyl and 3,4-dehydroproline, are shown to block the production of agglutinin activity in an in vivo bioassay system. These results indicate that the agglutinin glycoprotein may be related to a class of hydroxyproline-rich glycoproteins found in the extracellular matrix of higher plants, several of which have been shown to have lectin activity.

Citing Articles

Monoclonal antibodies to the major structural glycoprotein of the Chlamydomonas cell wall.

Smith E, Roberts K, Hutchings A, Galfre G Planta. 2013; 161(4):330-8.

PMID: 24253722 DOI: 10.1007/BF00398723.


Evidence for a functional membrane barrier in the transition zone between the flagellum and cell body of Chlamydomonas eugametos gametes.

Musgrave A, de Wildt P, van Etten I, Pijst H, Scholma C, Kooyman R Planta. 2013; 167(4):544-53.

PMID: 24240371 DOI: 10.1007/BF00391231.


Light dependence of sexual agglutinability in Chlamydomonas eugametos.

Kooijman R, Elzenga T, de Wildt P, Musgrave A, Schuring F, van den Ende H Planta. 2013; 169(3):370-8.

PMID: 24232649 DOI: 10.1007/BF00392133.


Identification of a highly conserved hydroxyproline-rich glycoprotein in the cell walls of Chlamydomonas reinhardtii and two other Volvocales.

Adair W, Appel H Planta. 2013; 179(3):381-6.

PMID: 24201668 DOI: 10.1007/BF00391084.


Characterization of native and modified extensin monomers and oligomers by electron microscopy and gel filtration.

Heckman J, Terhune B, Lamport D Plant Physiol. 1988; 86(3):848-56.

PMID: 16665999 PMC: 1054582. DOI: 10.1104/pp.86.3.848.


References
1.
Sadava D, Chrispeels M . Hydroxyproline biosynthesis in plant cells. Peptidyl proline hydroxylase from carrot disks. Biochim Biophys Acta. 1971; 227(2):278-87. DOI: 10.1016/0005-2744(71)90060-x. View

2.
Goodenough U, WEISS R . Gametic differentiation in Chlamydomonas reinhardtii. III. Cell wall lysis and microfilament-associated mating structure activation in wild-type and mutant strains. J Cell Biol. 1975; 67(3):623-37. PMC: 2111651. DOI: 10.1083/jcb.67.3.623. View

3.
Mecham R, LANGE G . Antigenicity of elastin: characterization of major antigenic determinants on purified insoluble elastin. Biochemistry. 1982; 21(4):669-73. DOI: 10.1021/bi00533a013. View

4.
Brown W, Howard G . Amino acid composition of proteins eluted from polyacrylamide gels: background considerations. Anal Biochem. 1980; 101(2):294-8. DOI: 10.1016/0003-2697(80)90189-x. View

5.
Tanaka M, Shibata H, Uchida T . A new prolyl hydroxylase acting on poly-L-proline, from suspension cultured cells of Vinca rosea. Biochim Biophys Acta. 1980; 616(2):188-98. DOI: 10.1016/0005-2744(80)90137-0. View