Localization of Deformed Wing Virus Infection in Queen and Drone Apis Mellifera L

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2006 Mar 30

PMID 16569216

Citations 44

Authors

Julie Fievet

Diana Tentcheva

Laurent Gauthier

Joachim de Miranda

Francois Cousserans

Marc Edouard Colin

Max Bergoin

Affiliations

Soon will be listed here.

Abstract

The distribution of deformed wing virus infection within the honey bee reproductive castes (queens, drones) was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3' portion of the DWV genome were used to identify DWV RNA, while a monospecific antibody to the DWV-VP1 structural protein was used to identify viral proteins and particles. The histological data were confirmed by quantitative RT-PCR of dissected organs. Results showed that DWV infection is not restricted to the digestive tract of the bee but spread in the whole body, including queen ovaries, queen fat body and drone seminal vesicles.

Citing Articles

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Lopes A, Low M, Martin-Hernandez R, Pinto M, de Miranda J Virus Evol. 2024; 10(1):veae053.

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Deformed wing virus of honey bees is inactivated by cold plasma ionized hydrogen peroxide.

Cook S, Ryabov E, Becker C, Rogers C, Posada-Florez F, Evans J Front Insect Sci. 2024; 3:1216291.

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